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Literature summary for 2.3.1.37 extracted from

  • Gong, J.; Hunter, G.A.; Ferreira, G.C.
    Aspartate-279 in aminolevulinate synthase affects enzyme catalysis through enhancing the function of the pyridoxal 5'-phosphate cofactor (1998), Biochemistry, 37, 3509-3517.
    View publication on PubMed
Search for more literature for 2.3.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expression of erythroid-specific isoform ALAS-E and mutants in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
D279A exchange mutant of potential cofactor binding residue Asp279, no activity, dissociation constant for pyridoxal 5'-phosphate is 19fold increased, different mode of cofactor binding, no formation of quinonoid reaction intermediate, which can be restored by addition of analogue N-methyl-pyridoxal 5'-phosphate Mus musculus
D279E exchange mutant of potential cofactor binding residue Asp279, 30fold reduced catalytic efficiency for succinyl-CoA compared to the wild-type Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 succinyl-CoA recombinant erythroid isoform wild-type Mus musculus
0.035
-
 succinyl-CoA recombinant erythroid isoform mutant D279E Mus musculus
23
-
 glycine recombinant erythroid isoform wild-type Mus musculus
140
-
 glycine recombinant erythroid isoform mutant D279E Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 erythroid-specific isoform
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutants from E. coli Mus musculus

Reaction

Reaction Comment Organism Reaction ID
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation Mus musculus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0967
-
 succinyl-CoA recombinant erythroid isoform mutant D279E Mus musculus
0.167
-
 succinyl-CoA recombinant erythroid isoform Mus musculus

Cofactor

Cofactor Comment Organism Structure
N-methyl-pyridoxal 5'-phosphate in vitro, mutant D279A Mus musculus
pyridoxal 5'-phosphate required Mus musculus