Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged wild-type and mutants in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
Y121F | exchange mutant of potential cofactor binding residue Tyr121, 5% activity compared to the wild-type, Km for glycine is 5fold increased, lower affinity for pyridoxal 5'phosphate | Mus musculus |
Y121H | exchange mutant of potential cofactor binding residue Tyr121, 36% activity compared to the wild-type, Km for glycine is 34fold increased, lower affinity for pyridoxal 5'phosphate | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
succinyl-CoA | recombinant mutant Y121H | Mus musculus | |
0.002 | - |
succinyl-CoA | recombinant erythroid isoform wild-type | Mus musculus | |
0.014 | - |
succinyl-CoA | recombinant mutant Y121F | Mus musculus | |
11.7 | - |
glycine | recombinant erythroid isoform | Mus musculus | |
52.2 | - |
glycine | recombinant mutant Y121H | Mus musculus | |
400 | - |
glycine | recombinant mutant Y121F | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence alignment | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
erythroid-specific isoform | - |
Purification (Comment) | Organism |
---|---|
recombinant of His-tagged wild-type and mutants from E. coli | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0355 | - |
succinyl-CoA | recombinant erythroid isoform mutant Y121H | Mus musculus | |
0.235 | - |
succinyl-CoA | recombinant erythroid isoform mutant Y121F | Mus musculus | |
0.658 | - |
succinyl-CoA | recombinant erythroid isoform | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required | Mus musculus |