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Literature summary for 2.3.1.37 extracted from

  • Dailey, H.A.; Dailey, T.A.
    Expression and purification of mammalian 5-aminolevulinate synthase (1997), Methods Enzymol., 281, 336-340.
    View publication on PubMed
Search for more literature for 2.3.1.37

General Stability

General Stability Organism
short half-life Gallus gallus
short half-life Mus musculus
short half-life Homo sapiens
short half-life Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
protoheme feed-back inhibition, non-specific isoform ALAS-1 Gallus gallus
protoheme feed-back inhibition, non-specific isoform ALAS-1 Homo sapiens
protoheme feed-back inhibition, non-specific isoform ALAS-1 Mus musculus
protoheme feed-back inhibition, non-specific isoform ALAS-1 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
 succinyl-CoA
-
 Mus musculus
51
-
 glycine
-
 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE Gallus gallus
Iron regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE Mus musculus
Iron regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE Homo sapiens
Iron regulation of erythroid-specific isoform ALAS-2 via 5'-iron responsive element, i.e. IRE Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 2 * 56000, SDS-PAGE Mus musculus
112000
-
 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme deficiency causes X-linked sideroblastic anemia ?
-
 ?
succinyl-CoA + glycine Gallus gallus key enzyme in tetrapyrrole biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine Mus musculus key enzyme in tetrapyrrole biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine Homo sapiens key enzyme in tetrapyrrole biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine Rattus norvegicus key enzyme in tetrapyrrole biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 2 isoforms ALAS-1 and ALAS-2, a houskeeping form and an erythroid-specific form
-
Homo sapiens
-
 2 isoforms ALAS-1 and ALAS-2, a houskeeping form and an erythroid-specific form
-
Mus musculus
-
 2 isoforms ALAS-1 and ALAS-2, a houskeeping form and an erythroid-specific form
-
Rattus norvegicus
-
 non-specific, housekeeping isoform ALAS-N and erythroid-specific isoform ALAS-E
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from E. coli Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence Gallus gallus
-
additional information enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence Mus musculus
-
additional information enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence Homo sapiens
-
additional information enzyme is synthezised in the cytosol as the precursor protein and then imported into the mitochondria matrix and cleaved to the mature enzyme, the targeting information is encoded in nonoverlapping regions of the presequence Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme deficiency causes X-linked sideroblastic anemia Homo sapiens ?
-
 ?
succinyl-CoA + glycine
-
 Gallus gallus 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine
-
 Homo sapiens 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine
-
 Rattus norvegicus 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine key enzyme in tetrapyrrole biosynthesis Gallus gallus 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine key enzyme in tetrapyrrole biosynthesis Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine key enzyme in tetrapyrrole biosynthesis Homo sapiens 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine key enzyme in tetrapyrrole biosynthesis Rattus norvegicus 5-aminolevulinate + CoA + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Gallus gallus
dimer
-
 Rattus norvegicus
dimer 2 * 56000, SDS-PAGE Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0095
-
 succinyl-CoA
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required Gallus gallus
pyridoxal 5'-phosphate required Mus musculus
pyridoxal 5'-phosphate required Homo sapiens
pyridoxal 5'-phosphate required Rattus norvegicus
pyridoxal 5'-phosphate noncovalently bound Mus musculus