Inhibitors | Comment | Organism | Structure |
---|---|---|---|
divalent cations | Mg2+ or Ca2+: optimal at concentration of 50 mM, inhibition above 200 mM | Gallus gallus | |
hemin | - |
Gallus gallus | |
monovalent cations | Na+ or Li+: optimal at 0.5 M, not inhibitory up to 1 M | Gallus gallus | |
p-chloromercuribenzoate | - |
Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | optimal at concentration of 50 mM, inhibition above 200 mM | Gallus gallus | |
Li+ | optimal at 0.5 M, not inhibitory up to 1 M | Gallus gallus | |
Li+ | in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory | Gallus gallus | |
Mg2+ | optimal at concentration of 50 mM, inhibition above 200 mM | Gallus gallus | |
Na+ | optimal at 0.5 M, not inhibitory up to 1 M | Gallus gallus | |
Na+ | in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
2 * 49000, SDS-PAGE | Gallus gallus |
87000 | - |
gel filtration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
liver | fetal | Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Gallus gallus |
Storage Stability | Organism |
---|---|
-15°C, 20 mM potassium phosphate buffer, pH 7.6, 1 mM dithioerythritol, 0.01 mM pyridoxal 5'-phosphate, 30% loss of activity after 2 weeks | Gallus gallus |
4°C, 20 mM potassium phosphate buffer, pH 7.6, 1 mM dithioerythritol, 0.01 mM pyridoxal 5'-phosphate, 20% loss of activity after 2 weeks | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Gallus gallus | 5-aminolevulinate + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49000, SDS-PAGE | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required | Gallus gallus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
hemin | - |
Gallus gallus |