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Literature summary for 2.3.1.37 extracted from

  • Whiting, M.J.; Granick, S.
    delta-Aminolevulinic acid synthase from chick embryo liver mitochondria. I. Purification and some properties (1976), J. Biol. Chem., 251, 1340-1346.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
divalent cations Mg2+ or Ca2+: optimal at concentration of 50 mM, inhibition above 200 mM Gallus gallus
hemin
-
Gallus gallus
monovalent cations Na+ or Li+: optimal at 0.5 M, not inhibitory up to 1 M Gallus gallus
p-chloromercuribenzoate
-
Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ optimal at concentration of 50 mM, inhibition above 200 mM Gallus gallus
Li+ optimal at 0.5 M, not inhibitory up to 1 M Gallus gallus
Li+ in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory Gallus gallus
Mg2+ optimal at concentration of 50 mM, inhibition above 200 mM Gallus gallus
Na+ optimal at 0.5 M, not inhibitory up to 1 M Gallus gallus
Na+ in Mg2+-containing medium: at 100 mM no effect on activity, higher concentrations are totally inhibitory Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
2 * 49000, SDS-PAGE Gallus gallus
87000
-
gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Gallus gallus
-
liver fetal Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Gallus gallus

Storage Stability

Storage Stability Organism
-15°C, 20 mM potassium phosphate buffer, pH 7.6, 1 mM dithioerythritol, 0.01 mM pyridoxal 5'-phosphate, 30% loss of activity after 2 weeks Gallus gallus
4°C, 20 mM potassium phosphate buffer, pH 7.6, 1 mM dithioerythritol, 0.01 mM pyridoxal 5'-phosphate, 20% loss of activity after 2 weeks Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
Gallus gallus 5-aminolevulinate + CoA + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 49000, SDS-PAGE Gallus gallus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.035
-
hemin
-
Gallus gallus