Literature summary for 2.3.1.35 extracted from

Hao, N.; Mu, J.; Hu, N.; Xu, S.; Shen, P.; Yan, M.; Li, Y.; Xu, L.
Implication of ornithine acetyltransferase activity on L-ornithine production in Corynebacterium glutamicum (2016), Biotechnol. Appl. Biochem., 63, 15-21.

Search for more literature for 2.3.1.35

Cloned(Commentary)

Cloned (Comment)	Organism
gene argJ, cloning in Escherichia coli strain DH5alpha, recombinant overexpression in Corynebacterium glutamicum strain 1006	Corynebacterium glutamicum

Protein Variants

Protein Variants	Comment	Organism
additional information	inactivation of regulatory repressor argR gene and overexpression of argJ gene, argJ overexpression leads to decreased ornithine acetyltransferase activity due to product inhibition by L-ornithine	Corynebacterium glutamicum

Inhibitors

Inhibitors	Comment	Organism	Structure
L-ornithine	product inhibition	Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N2-acetyl-L-ornithine + L-glutamate	Corynebacterium glutamicum	-	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	Corynebacterium glutamicum ATCC 13032	-	L-ornithine + N-acetyl-L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	Q59280	gene argJ	-
Corynebacterium glutamicum ATCC 13032	Q59280	gene argJ	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.004	-	enzyme activity in argJ-overexpressing strain 1006, pH 7.5, 30°C	Corynebacterium glutamicum
0.008	-	enzyme activity in mutant strain 1006DELTAargR, pH 7.5, 30°C	Corynebacterium glutamicum
0.015	-	enzyme activity in mutant strain 1006DELTAargR-DELTAargJ, pH 7.5, 30°C	Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N2-acetyl-L-ornithine + L-glutamate	-	Corynebacterium glutamicum	L-ornithine + N-acetyl-L-glutamate	-	?
N2-acetyl-L-ornithine + L-glutamate	-	Corynebacterium glutamicum ATCC 13032	L-ornithine + N-acetyl-L-glutamate	-	?

Synonyms

Synonyms	Comment	Organism
argJ	-	Corynebacterium glutamicum
OATase	-	Corynebacterium glutamicum
ornithine acetyltransferase	-	Corynebacterium glutamicum

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
5	-	pH 7.5, 30°C	Corynebacterium glutamicum	L-ornithine

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Release 2023.1 (January 2023)