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Literature summary for 2.3.1.35 extracted from

  • Haskins, N.; Panglao, M.; Qu, Q.; Majumdar, H.; Cabrera-Luque, J.; Morizono, H.; Tuchman, M.; Caldovic, L.
    Inversion of allosteric effect of arginine on N-acetylglutamate synthase, a molecular marker for evolution of tetrapods (2008), BMC Biochem., 9, 24.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.35

Activating Compound

Activating Compound Comment Organism Structure
L-arginine
-
 Mus musculus

Protein Variants

Protein Variants Comment Organism
E280A change of resiude to the corresponding Escherichia coli residue. Mutation abolishes arginine inhibition and decreases synthase activity Xanthomonas campestris
E354A change of residue to the corresponding Escherichia coli residue. Mutation abolishes arginine activation Mus musculus
F121C change of residue to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine activation Mus musculus
F35C change of resiude to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation leads to partial inhibition of both synthase and kinase activities by arginine and decrease in synthase activity Xanthomonas campestris
G286P change of resiude to the corresponding Escherichia coli residue. Mutation abolishes arginine inhibition and decreases synthase activity Xanthomonas campestris
G288S change of resiude to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine inhibition and decreases synthase activity Xanthomonas campestris
G360P change of residue to the corresponding Escherichia coli residue. Mutation abolishes arginine activation Mus musculus
G362S change of residue to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine activation Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
arginine
-
 Xanthomonas campestris

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Xanthomonas campestris
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2
-
 mutant G362S Mus musculus
3.2
-
 mutant G362S, presence of 1 mM L-arginine Mus musculus
3.4
-
 mutant G286P, presence of 1 mM L-arginine Xanthomonas campestris
3.5
-
 mutant G286P Xanthomonas campestris
4.3
-
 mutant F35C, presence of 1 mM L-arginine Xanthomonas campestris
9.7
-
 mutant F35C Xanthomonas campestris
12.2
-
 mutant F121C Mus musculus
12.3
-
 wild-type Mus musculus
12.3
-
 wild-type, presence of 1 mM L-arginine Mus musculus
12.6
-
 mutant G360P, presence of 1 mM L-arginine Mus musculus
13
-
 mutant G360P Mus musculus
13.1
-
 mutant E354A, presence of 1 mM L-arginine Mus musculus
15.3
-
 mutant F121C, presence of 1 mM L-arginine Mus musculus
15.5
-
 mutant E354A Mus musculus
26.7
-
 mutant E280A, presence of 1 mM L-arginine Xanthomonas campestris
26.9
-
 mutant E280A Xanthomonas campestris
42.6
-
 mutant G288S, presence of 1 mM L-arginine Xanthomonas campestris
46
-
 mutant G288S Xanthomonas campestris
71
-
 wild-type Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N2-acetyl-L-ornithine + L-glutamate
-
 Mus musculus L-ornithine + N-acetyl-L-glutamate
-
 ?
N2-acetyl-L-ornithine + L-glutamate
-
 Xanthomonas campestris L-ornithine + N-acetyl-L-glutamate
-
 ?