Cloned (Comment) | Organism |
---|---|
- |
Citrullus lanatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by citrulline or arginine at physiologically relevant high concentrations | Citrullus lanatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
N2-Acetyl-L-ornithine | pH 7.0 | Citrullus lanatus | |
17.8 | - |
L-glutamate | pH 7.0 | Citrullus lanatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21300 | - |
2 * 21300 + 2 * 23500, calculated from sequence | Citrullus lanatus |
23500 | - |
2 * 21300 + 2 * 23500, calculated from sequence | Citrullus lanatus |
90000 | - |
gel filtration | Citrullus lanatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N2-acetyl-L-ornithine + L-glutamate | Citrullus lanatus | CLGAT can effectively participate in the biosynthesis of citrulline in leaves of during drought/strong-light stress | L-ornithine + N-acetyl-L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrullus lanatus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the two subunits withj molecular masses of 21300 Da and 23500 Da are derived from a single precursor polypeptide, suggesting that the CLGAT precursor is cleaved autocatalytically at the conserved ATML motif. The first 26-amino acid sequence at the N-terminus of the precursor functions as a chloroplast transit peptide | Citrullus lanatus |
Purification (Comment) | Organism |
---|---|
- |
Citrullus lanatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Citrullus lanatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.7 | - |
above | Citrullus lanatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N2-acetyl-L-ornithine + L-glutamate | - |
Citrullus lanatus | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | CLGAT can effectively participate in the biosynthesis of citrulline in leaves of during drought/strong-light stress | Citrullus lanatus | L-ornithine + N-acetyl-L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 21300 + 2 * 23500, calculated from sequence | Citrullus lanatus |
Synonyms | Comment | Organism |
---|---|---|
CLGAT | - |
Citrullus lanatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Citrullus lanatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | 40°C: about 40% of maximal activity, 80°C: about 45% of maximal activity | Citrullus lanatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable up to | Citrullus lanatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Citrullus lanatus |