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Literature summary for 2.3.1.33 extracted from

  • Yamada, S.; Arikawa, S.
    An ectotherm homologue of human predicted gene NAT16 encodes histidine N-acetyltransferase responsible for Nalpha-acetylhistidine synthesis (2014), Biochim. Biophys. Acta, 1840, 434-442.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene hisat, DNA and amino acid sequence determination and analysis Oreochromis niloticus
gene hisat, DNA and amino acid sequence determination and analysis, the HISAT gene is expressed as two splice variants containing an identical ORF but differing lengths of 5'-UTR. Both variants are expressed exclusively in the fish brain and lens, recombinant expression in a cell-free translation system, and recombinant expression of the two HISAT splice variant-1 and -2 in Oreochromis niloticus Scomber australasicus
gene Nat16, DNA and amino acid sequence determination and analysis, recombinant expression in a cell-free translation system Homo sapiens

General Stability

General Stability Organism
HISAT is an unstable enzyme that is rapidly and irreversibly inactivated during preincubation at 37 °C in the absence of acetyl-CoA Scomber australasicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
gel filtration Scomber australasicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-histidine Scomber australasicus
-
CoA + N-acetyl-L-histidine
-
?
acetyl-CoA + L-histidine Homo sapiens
-
CoA + N-acetyl-L-histidine
-
?
acetyl-CoA + L-histidine Oreochromis niloticus
-
CoA + N-acetyl-L-histidine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8N8M0 gene Nat16
-
Oreochromis niloticus I3J7Q8 gene hisat, two HISAT splice variant-1 and -2
-
Scomber australasicus U3U715 gene hisat, two HISAT splice variant-1 and -2
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 4453fold from brain by ammonium sulfate fractionation, dye-ligand affinity chromatography, gel filtration, and anion exchange chromatography followed by ultrafiltration Scomber australasicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Scomber australasicus
-
brain
-
Homo sapiens
-
lens
-
Scomber australasicus
-
additional information the HISAT gene is expressed as two splice variants containing an identical ORF but differing lengths of 5'-UTR. Both variants are expressed exclusively in the fish brain and lens Scomber australasicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1692
-
purified native enzyme, pH 7.8, 37°C Scomber australasicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-histidine
-
Scomber australasicus CoA + N-acetyl-L-histidine
-
?
acetyl-CoA + L-histidine
-
Homo sapiens CoA + N-acetyl-L-histidine
-
?
acetyl-CoA + L-histidine
-
Oreochromis niloticus CoA + N-acetyl-L-histidine
-
?
additional information the recombinant human NAT16 protein has only trace enzyme activity for N-acetyl-L-histidine synthesis Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
HISAT
-
Scomber australasicus
HISAT
-
Homo sapiens
HISAT
-
Oreochromis niloticus
N-acetyltransferase 16
-
Homo sapiens
N-acetyltransferase 16
-
Oreochromis niloticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Scomber australasicus
37
-
assay at Homo sapiens
37
-
assay at Oreochromis niloticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
the purified enzyme HISAT is an unstable enzyme that is rapidly and irreversibly inactivated during preincubation at 37 °C in the absence of acetyl-CoA Scomber australasicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Scomber australasicus
7.8
-
assay at Homo sapiens
7.8
-
assay at Oreochromis niloticus

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Scomber australasicus
acetyl-CoA
-
Homo sapiens
acetyl-CoA
-
Oreochromis niloticus

General Information

General Information Comment Organism
additional information partial amino acid sequences determination and analysis using mass spectrometry and Edman degradation Scomber australasicus
additional information partial amino acid sequences determination and analysis using mass spectrometry and Edman degradation Homo sapiens