Cloned (Comment) | Organism |
---|---|
gene hisat, DNA and amino acid sequence determination and analysis | Oreochromis niloticus |
gene hisat, DNA and amino acid sequence determination and analysis, the HISAT gene is expressed as two splice variants containing an identical ORF but differing lengths of 5'-UTR. Both variants are expressed exclusively in the fish brain and lens, recombinant expression in a cell-free translation system, and recombinant expression of the two HISAT splice variant-1 and -2 in Oreochromis niloticus | Scomber australasicus |
gene Nat16, DNA and amino acid sequence determination and analysis, recombinant expression in a cell-free translation system | Homo sapiens |
General Stability | Organism |
---|---|
HISAT is an unstable enzyme that is rapidly and irreversibly inactivated during preincubation at 37 °C in the absence of acetyl-CoA | Scomber australasicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
gel filtration | Scomber australasicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-histidine | Scomber australasicus | - |
CoA + N-acetyl-L-histidine | - |
? | |
acetyl-CoA + L-histidine | Homo sapiens | - |
CoA + N-acetyl-L-histidine | - |
? | |
acetyl-CoA + L-histidine | Oreochromis niloticus | - |
CoA + N-acetyl-L-histidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8N8M0 | gene Nat16 | - |
Oreochromis niloticus | I3J7Q8 | gene hisat, two HISAT splice variant-1 and -2 | - |
Scomber australasicus | U3U715 | gene hisat, two HISAT splice variant-1 and -2 | - |
Purification (Comment) | Organism |
---|---|
native enzyme 4453fold from brain by ammonium sulfate fractionation, dye-ligand affinity chromatography, gel filtration, and anion exchange chromatography followed by ultrafiltration | Scomber australasicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Scomber australasicus | - |
brain | - |
Homo sapiens | - |
lens | - |
Scomber australasicus | - |
additional information | the HISAT gene is expressed as two splice variants containing an identical ORF but differing lengths of 5'-UTR. Both variants are expressed exclusively in the fish brain and lens | Scomber australasicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1692 | - |
purified native enzyme, pH 7.8, 37°C | Scomber australasicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-histidine | - |
Scomber australasicus | CoA + N-acetyl-L-histidine | - |
? | |
acetyl-CoA + L-histidine | - |
Homo sapiens | CoA + N-acetyl-L-histidine | - |
? | |
acetyl-CoA + L-histidine | - |
Oreochromis niloticus | CoA + N-acetyl-L-histidine | - |
? | |
additional information | the recombinant human NAT16 protein has only trace enzyme activity for N-acetyl-L-histidine synthesis | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HISAT | - |
Scomber australasicus |
HISAT | - |
Homo sapiens |
HISAT | - |
Oreochromis niloticus |
N-acetyltransferase 16 | - |
Homo sapiens |
N-acetyltransferase 16 | - |
Oreochromis niloticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Scomber australasicus |
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Oreochromis niloticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the purified enzyme HISAT is an unstable enzyme that is rapidly and irreversibly inactivated during preincubation at 37 °C in the absence of acetyl-CoA | Scomber australasicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Scomber australasicus |
7.8 | - |
assay at | Homo sapiens |
7.8 | - |
assay at | Oreochromis niloticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Scomber australasicus | |
acetyl-CoA | - |
Homo sapiens | |
acetyl-CoA | - |
Oreochromis niloticus |
General Information | Comment | Organism |
---|---|---|
additional information | partial amino acid sequences determination and analysis using mass spectrometry and Edman degradation | Scomber australasicus |
additional information | partial amino acid sequences determination and analysis using mass spectrometry and Edman degradation | Homo sapiens |