Literature summary for 2.3.1.32 extracted from

Ho, P.C.; Gupta, P.; Tsui, Y.C.; Ha, S.G.; Huq, M.; Wei, L.N.
Modulation of lysine acetylation-stimulated repressive activity by Erk2-mediated phosphorylation of RIP140 in adipocyte differentiation (2008), Cell. Signal., 20, 1911-1919.

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the phosphorylation of the receptor-interacting protein 140 (RIP140) by extracellular-signal-related kinase 2 (Erk2) stimulates p300 acetyltransferase to acetylate the RIP140's lysine 158 and lysine 287 residues	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
transfection of COS-1 cells (African green monkey cell line) with plasmids of wild-type enzyme, deficient enzyme or single site mutants fused to reporter genes	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	Lysine 158 and 287 in the RIP140 (receptor-interacting protein 140) are targets for acetylation by p300 acetyltransferase as shown by site directed mutagenesis of these residues, lysine 158 and 287 replaced by glutamine (mimicking acetylation) turn the RIP140 repressive in the absence of p300, while the alanine replacements (preventing acetylation) prevents responsiveness to p300, the double mutants show stronger effects in the same directions, while lysine 111 and 311 seem not to be targets for the p300 acetyltransferase. Mitogen-activated protein kinase (MAPK) target mutants that mimic (T202E/T207E) or abolish (T202A/T207A) threonine phosphorylation of RIP140 enhance or reduce the stimulation of p300 acetyltransferase, respectively, extracellular-signal-related kinase 2 (Erk2) is identified as the responsible MAPK in this pathway	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
anacardic acid	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + L-lysine	Homo sapiens	-	CoA + N6-acetyl-L-lysine	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
cells are washed with PBS, harvested in Tris-HCl, pH 7.4, containing 0.5% deoxycholic acid, 150 mM NaCl, 0.1% SDS, 4 mM EDTA, and 1% NP-40, with a protease inhibitor cocktail, 1 mM PMSF, 1 mM sodium fluoride, and 1 mM sodium orthovanadate, centrifugation, supernatant subjected to SDS-PAGE, or for immunoprecipitation lysed cells are collected with 50 mM Tris-HCl, pH 8.0, with 10% glycerol, 100 mM NaCl, 1 mM EDTA, and 0.1% NP-40 with proteinase inhibitor cocktail, 1 mM PMSF, 1 mM sodium fluoride, and 1 mM sodium orthovanadate, incubation with antibodies and G beads, washing, elution of proteins by boiling in 2X Laemmli loading buffer	Homo sapiens

Purification (Comment)

Organism

cells are washed with PBS, harvested in Tris-HCl, pH 7.4, containing 0.5% deoxycholic acid, 150 mM NaCl, 0.1% SDS, 4 mM EDTA, and 1% NP-40, with a protease inhibitor cocktail, 1 mM PMSF, 1 mM sodium fluoride, and 1 mM sodium orthovanadate, centrifugation, supernatant subjected to SDS-PAGE, or for immunoprecipitation lysed cells are collected with 50 mM Tris-HCl, pH 8.0, with 10% glycerol, 100 mM NaCl, 1 mM EDTA, and 0.1% NP-40 with proteinase inhibitor cocktail, 1 mM PMSF, 1 mM sodium fluoride, and 1 mM sodium orthovanadate, incubation with antibodies and G beads, washing, elution of proteins by boiling in 2X Laemmli loading buffer

Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + L-lysine	-	Homo sapiens	CoA + N6-acetyl-L-lysine	-	?

Synonyms

Synonyms	Comment	Organism
lysine acetyltransferase	-	Homo sapiens
p300 acetyltransferase	-	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	specific lysine 158 and lysine 287 acetylation of RIP140 (receptor-interacting protein 140) which is a co-regulator for many transcription factors, acetylation directly enhances the regulator's trans-repressive activity, role in fat accumulation	Homo sapiens