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Literature summary for 2.3.1.30 extracted from

  • Fu, J.; Zhang, X.; Chen, X.; Yin, Y.; Ma, Z.
    Serine O-acetyltransferase is important, but not essential for cysteine-methionine synthesis in Fusarium graminearum (2014), World J. Microbiol. Biotechnol., 30, 1219-1228.
    View publication on PubMed
Search for more literature for 2.3.1.30

Cloned(Commentary)

Cloned (Comment) Organism
gene FgSAT or FGSG_00186, DNA and amino acid sequence determination and analysisphylogenetic analysis and tree, real-time PCR expression analysis, genetic complementation of FgSAT deletion mutant Fusarium graminearum

Protein Variants

Protein Variants Comment Organism
additional information generation of three deletion mutants of FgSAT, DELTAFgSAT-1, -2 and -18, using a gene replacement strategy. The three mutants show no recognizable phenotypic changes on potato dextrose agar medium, but exhibit a very weak growth on fructose gelatin agar medium containing sulfate as sole sulfur source. Supplementation of O-acetylserine, cysteine, or methionine, but not serine, rescues the defect of mycelial growth in FgSAT deletion mutants. The three mutants have a decrease in conidiation in mung bean liquid, but not in carboxymethyl cellulose. The mutant virulence, deoxynivalenol production and fungicide sensitivity is not altered compared to the wild-type. Real-time PCR assays detect an increase in expression levels of cysteine synthase (FgOAS), cystathionine gamma-synthase (FgCGS), cystathionine beta-lyase (FgCBL) genes involved in the alternative pathway in FgSAT deletion mutants, suggesting that the alternative pathway in Fusarium graminearum is present and can operate. Phenotype, overview Fusarium graminearum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-serine Fusarium graminearum
-
 CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine Fusarium graminearum PH-1
-
 CoA + O-acetyl-L-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium graminearum I1R9N5 gene FGSG_00186
-
Fusarium graminearum PH-1 I1R9N5 gene FGSG_00186
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Fusarium graminearum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-serine
-
 Fusarium graminearum CoA + O-acetyl-L-serine
-
 ?
acetyl-CoA + L-serine
-
 Fusarium graminearum PH-1 CoA + O-acetyl-L-serine
-
 ?

Synonyms

Synonyms Comment Organism
SAT
-
 Fusarium graminearum

General Information

General Information Comment Organism
metabolism serine O-acetyltransferase (SAT) is a key enzyme converting serine into O-acetylserine in the synthesis of sulfur-containing amino acids Fusarium graminearum