Literature summary for 2.3.1.30 extracted from

McManus, M.T.; Leung, S.; Lambert, A.; Scott, R.W.; Pither-Joyce, M.; Chen, B.; McCallum, J.
Molecular and biochemical characterisation of a serine acetyltransferase of onion, Allium cepa (L.) (2005), Phytochemistry, 66, 1407-1416.

Search for more literature for 2.3.1.30

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli as a fusion protein with glutathione-S-transferase	Allium cepa

Inhibitors

Inhibitors	Comment	Organism	Structure
L-cysteine	IC50: 0.0031 mM	Allium cepa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.092	-	acetyl-CoA	pH 8.0, 25°C	Allium cepa
0.72	-	L-serine	pH 8.0, 25°C	Allium cepa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Allium cepa	9507	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	x * 33000, SDS-PAGE	Allium cepa

Organism

Organism	UniProt	Comment	Textmining
Allium cepa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Allium cepa

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	SAT1 gene is induced in leaf tissue in response ti low S-supply	Allium cepa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + L-serine	-	Allium cepa	CoA + O-acetyl-L-serine	-	?

Subunits

Subunits	Comment	Organism
?	x * 33000, SDS-PAGE	Allium cepa

Synonyms

Synonyms	Comment	Organism
SAT1	-	Allium cepa

pI Value

Organism	Comment	pI Value Maximum	pI Value
Allium cepa	calculation from nucleotide sequence	-	6.52

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0031	-	IC50: 0.0031 mM	Allium cepa	L-cysteine

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)