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Literature summary for 2.3.1.30 extracted from

  • Kobayashi, S.; Masui, R.; Yokoyama, S.; Kuramitsu, S.; Takagi, H.
    A novel metal-activated L-serine O-acetyltransferase from Thermus thermophilus HB8 (2004), J. Biochem., 136, 629-634.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermus thermophilus HB8

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1.0 mM, complete inhibition Thermus thermophilus HB8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
acetyl-CoA pH 7.8, 30°C Thermus thermophilus HB8
0.013
-
L-serine pH 7.8, 30°C Thermus thermophilus HB8

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.1 mM, 31.7fold activation Thermus thermophilus HB8
Fe2+ enzyme contains Zn2+ and Fe2+ Thermus thermophilus HB8
Ni2+ 0.1 mM, 12fold activation Thermus thermophilus HB8
Zn2+ enzyme contains Zn2+ and Fe2+ Thermus thermophilus HB8

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, SDS-PAGE Thermus thermophilus HB8

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-Ser Thermus thermophilus HB8 key enzyme in L-cysteine biosynthesis CoA + O-acetyl-L-Ser
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus HB8 Q6F4D7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermus thermophilus HB8

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-Ser
-
Thermus thermophilus HB8 CoA + O-acetyl-L-Ser
-
?
acetyl-CoA + L-Ser key enzyme in L-cysteine biosynthesis Thermus thermophilus HB8 CoA + O-acetyl-L-Ser
-
?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Thermus thermophilus HB8

Synonyms

Synonyms Comment Organism
L-serine O-acetyltransferase
-
Thermus thermophilus HB8
SAT
-
Thermus thermophilus HB8

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Thermus thermophilus HB8

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 80 45°C: about 50% of maximal activity, 80°C: about 55% of maximal activity Thermus thermophilus HB8

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.48
-
L-serine pH 7.8, 30°C Thermus thermophilus HB8
0.54
-
acetyl-CoA pH 7.8, 30°C Thermus thermophilus HB8

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Thermus thermophilus HB8