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Literature summary for 2.3.1.30 extracted from
- Production of cysteine for bacterial and plant biotechnology: Application of cysteine feedback-insensitive isoforms of serine acetyltransferase (2003), Amino Acids, 24, 195-203.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in inactivated Escherichia coli mutant, insensitive against inhibition by L-cysteine | Nicotiana tabacum |
| expression in inactivated Escherichia coli mutant, unaltered L-cysteine sensitivity compared to wild-type | Nicotiana tabacum |
| insertional inactivation of wild-type CysE gene from Escherichia coli C600 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | - |
Escherichia coli |
| additional information | transformation via Agrobacterium tumefaciens, transgenic Nicotiana tabacum plants overexpressing recombinant Arabidopsis thaliana SAT A isozyme, expression with and without mitochondrial transit peptide | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-serine | Escherichia coli | - |
CoA + O-acetyl-L-serine | - |
? |  |
| acetyl-CoA + L-serine | Nicotiana tabacum | - |
CoA + O-acetyl-L-serine | - |
? | |
| acetyl-CoA + L-serine | Arabidopsis thaliana | - |
CoA + O-acetyl-L-serine | - |
? | |
| acetyl-CoA + L-serine | Escherichia coli C600 | - |
CoA + O-acetyl-L-serine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q39218 | - |
- |
| Arabidopsis thaliana | Q42538 | - |
- |
| Arabidopsis thaliana | Q42588 | - |
- |
| Escherichia coli | - |
CysE gene | - |
| Escherichia coli C600 | - |
CysE gene | - |
| Nicotiana tabacum | - |
cv. SNN | - |
| Nicotiana tabacum | Q8H0P5 | gene SAT7 | - |
| Nicotiana tabacum | Q8H0P6 | gene SAT4 | - |
| Nicotiana tabacum | Q8H0P7 | gene SAT1 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0025 | - |
wild-type strain C600 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-serine | - |
Escherichia coli | CoA + O-acetyl-L-serine | - |
? | |
| acetyl-CoA + L-serine | - |
Nicotiana tabacum | CoA + O-acetyl-L-serine | - |
? | |
| acetyl-CoA + L-serine | - |
Arabidopsis thaliana | CoA + O-acetyl-L-serine | - |
? | |
| acetyl-CoA + L-serine | - |
Escherichia coli C600 | CoA + O-acetyl-L-serine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SAT | - |
Escherichia coli |
| SAT | - |
Arabidopsis thaliana |
| SAT | - |
- |
| SAT | - |
Nicotiana tabacum |
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