Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli | Citrullus lanatus subsp. vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
E278L | site-directed mutagenesis, about 1.7fold more sensitive to inhibition by L-cysteine | Citrullus lanatus subsp. vulgaris |
F285Y | site-directed mutagenesis, about 1.7fold less sensitive to inhibition by L-cysteine | Citrullus lanatus subsp. vulgaris |
G277C | site-directed mutagenesis, about 27fold less sensitive to inhibition by L-cysteine | Citrullus lanatus subsp. vulgaris |
H282Q | site-directed mutagenesis, about 3.5fold less sensitive to inhibition by L-cysteine | Citrullus lanatus subsp. vulgaris |
additional information | SAT of transgenic Arabidopsis plants overexpressing the point-mutated watermelon gene are not inhibited by L-cysteine, regardless if the recombinant enzyme is targeted to the chloroplast or expressed in the cytosol | Arabidopsis thaliana |
additional information | SAT of transgenic Arabidopsis plants overexpressing the point-mutated watermelon gene are not inhibited by L-cysteine, regardless if the recombinant enzyme is targeted to the chloroplast or expressed in the cytosol | Citrullus lanatus subsp. vulgaris |
additional information | transgenic Arabidopsis plants expressing recombinant wild-type SATase are inhibited by L-cysteine | Arabidopsis thaliana |
additional information | transgenic Arabidopsis plants expressing recombinant wild-type SATase are inhibited by L-cysteine | Citrullus lanatus subsp. vulgaris |
S279T | site-directed mutagenesis, as sensitive to inhibition by L-cysteine as the wild-type | Citrullus lanatus subsp. vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | feed-back inhibition; weak | Allium tuberosum | |
L-cysteine | feed-back inhibition of cytosolic isozyme SAT-c, not of mitochondrial and plastidic isozymes SAT-m and SAT-p | Arabidopsis thaliana | |
L-cysteine | feed-back inhibition | Citrullus lanatus subsp. vulgaris | |
L-cysteine | feed-back inhibition | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Arabidopsis thaliana | |
additional information | - |
additional information | Km for L-serine and acetyl-CoA of recombinant L-cysteine-binding motif mutants, overview | Citrullus lanatus subsp. vulgaris | |
0.13 | - |
acetyl-CoA | - |
Arabidopsis thaliana | |
0.13 | - |
acetyl-CoA | - |
Citrullus lanatus subsp. vulgaris | |
0.59 | - |
L-serine | - |
Arabidopsis thaliana | |
0.59 | - |
L-serine | - |
Citrullus lanatus subsp. vulgaris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
chloroplast | - |
Citrullus lanatus subsp. vulgaris | 9507 | - |
chloroplast | - |
Allium tuberosum | 9507 | - |
cytosol | - |
Spinacia oleracea | 5829 | - |
cytosol | - |
Citrullus lanatus subsp. vulgaris | 5829 | - |
cytosol | - |
Allium tuberosum | 5829 | - |
cytosol | isozyme SAT-c | Arabidopsis thaliana | 5829 | - |
mitochondrion | - |
Citrullus lanatus subsp. vulgaris | 5739 | - |
mitochondrion | - |
Allium tuberosum | 5739 | - |
mitochondrion | isozyme SAT-m, matrix | Arabidopsis thaliana | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Spinacia oleracea | key role in the sulfur assimilatory pathway | CoA + O-acetyl-L-serine | - |
ir | |
acetyl-CoA + L-serine | Arabidopsis thaliana | key role in the sulfur assimilatory pathway | CoA + O-acetyl-L-serine | - |
ir | |
acetyl-CoA + L-serine | Citrullus lanatus subsp. vulgaris | key role in the sulfur assimilatory pathway | CoA + O-acetyl-L-serine | - |
ir | |
acetyl-CoA + L-serine | Allium tuberosum | key role in the sulfur assimilatory pathway | CoA + O-acetyl-L-serine | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Allium tuberosum | - |
Chinese chive | - |
Arabidopsis thaliana | - |
3 isozymes specific for subcellular compartment: SAT-c, SAT-m, SAT-p | - |
Citrullus lanatus subsp. vulgaris | - |
- |
- |
Spinacia oleracea | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Spinacia oleracea | CoA + O-acetyl-L-serine | - |
ir | |
acetyl-CoA + L-serine | - |
Arabidopsis thaliana | CoA + O-acetyl-L-serine | - |
ir | |
acetyl-CoA + L-serine | - |
Citrullus lanatus subsp. vulgaris | CoA + O-acetyl-L-serine | - |
ir | |
acetyl-CoA + L-serine | - |
Allium tuberosum | CoA + O-acetyl-L-serine | - |
ir | |
acetyl-CoA + L-serine | key role in the sulfur assimilatory pathway | Spinacia oleracea | CoA + O-acetyl-L-serine | - |
ir | |
acetyl-CoA + L-serine | key role in the sulfur assimilatory pathway | Arabidopsis thaliana | CoA + O-acetyl-L-serine | - |
ir | |
acetyl-CoA + L-serine | key role in the sulfur assimilatory pathway | Citrullus lanatus subsp. vulgaris | CoA + O-acetyl-L-serine | - |
ir | |
acetyl-CoA + L-serine | key role in the sulfur assimilatory pathway | Allium tuberosum | CoA + O-acetyl-L-serine | - |
ir |