Cloned (Comment) | Organism |
---|---|
expression of His-tagged protein encoded on gene cmSAT in Escherichia coli, functional complementation of SAT-deficient Escherichia coli strain JM15, DNA and amino acid sequence determination | Cyanidioschyzon merolae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | - |
Cyanidioschyzon merolae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | chloroplast isozyme, gene cmSAT | Cyanidioschyzon merolae | 9507 | - |
cytosol | small amount of enzyme | Cyanidioschyzon merolae | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, recombinant enzyme, SDS-PAGE | Cyanidioschyzon merolae |
43737 | - |
x * 43737, native enzyme, amino acid sequence determination | Cyanidioschyzon merolae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Cyanidioschyzon merolae | - |
CoA + O-acetyl-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyanidioschyzon merolae | - |
red alga | - |
Cyanidioschyzon merolae | - |
chloroplast isoform, constitutive gene cmSAT | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein from E. coli | Cyanidioschyzon merolae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
46 | - |
recombinant enzyme, crude extract | Cyanidioschyzon merolae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Cyanidioschyzon merolae | CoA + O-acetyl-L-serine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, recombinant enzyme, SDS-PAGE | Cyanidioschyzon merolae |
? | x * 43737, native enzyme, amino acid sequence determination | Cyanidioschyzon merolae |
Synonyms | Comment | Organism |
---|---|---|
More | serine acetyltransferase and O-acetylserine (thiol) lyase form the cysteine synthase complex | Cyanidioschyzon merolae |