Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.30 extracted from

  • Toda, K.; Takano, H.; Miyagishima, S.y.; Kuroiwa, H.; Kuroiwa, T.
    Characterization of a chloroplast isoform of serine acetyltransferase from the thermo-acidiphilic red alga Cyanidioschyzon merolae (1998), Biochim. Biophys. Acta, 1403, 72-84.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged protein encoded on gene cmSAT in Escherichia coli, functional complementation of SAT-deficient Escherichia coli strain JM15, DNA and amino acid sequence determination Cyanidioschyzon merolae

Inhibitors

Inhibitors Comment Organism Structure
L-cysteine
-
Cyanidioschyzon merolae

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast chloroplast isozyme, gene cmSAT Cyanidioschyzon merolae 9507
-
cytosol small amount of enzyme Cyanidioschyzon merolae 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 31000, recombinant enzyme, SDS-PAGE Cyanidioschyzon merolae
43737
-
x * 43737, native enzyme, amino acid sequence determination Cyanidioschyzon merolae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-serine Cyanidioschyzon merolae
-
CoA + O-acetyl-L-serine
-
?

Organism

Organism UniProt Comment Textmining
Cyanidioschyzon merolae
-
red alga
-
Cyanidioschyzon merolae
-
chloroplast isoform, constitutive gene cmSAT
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged protein from E. coli Cyanidioschyzon merolae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46
-
recombinant enzyme, crude extract Cyanidioschyzon merolae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-serine
-
Cyanidioschyzon merolae CoA + O-acetyl-L-serine
-
?

Subunits

Subunits Comment Organism
? x * 31000, recombinant enzyme, SDS-PAGE Cyanidioschyzon merolae
? x * 43737, native enzyme, amino acid sequence determination Cyanidioschyzon merolae

Synonyms

Synonyms Comment Organism
More serine acetyltransferase and O-acetylserine (thiol) lyase form the cysteine synthase complex Cyanidioschyzon merolae