Cloned (Comment) | Organism |
---|---|
expression of 3 isozymes SAT-m, SAT-p, SAT-c as GFP-fusion proteins in Arabidopsis thaliana | Arabidopsis thaliana |
overexpression of 3 isozymes SAT-m, SAT-p, SAT-c in Escherichia coli BL21 (DE3) | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | feed-back inhibition of cytosolic isozyme SAT-c, not of mitochondrial and plastidic isozymes SAT-m and SAT-p | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
acetyl-CoA | mitochondrial isozyme SAT-m | Arabidopsis thaliana | |
0.16 | - |
acetyl-CoA | chloroplastidic isozyme SAT-p | Arabidopsis thaliana | |
0.28 | - |
acetyl-CoA | cytosolic isozyme SAT-c | Arabidopsis thaliana | |
1.64 | - |
L-serine | chloroplastidic isozyme SAT-p | Arabidopsis thaliana | |
1.68 | - |
L-serine | mitochondrial isozyme SAT-m | Arabidopsis thaliana | |
2.71 | - |
L-serine | cytosolic isozyme SAT-c | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme SAT-p, stroma | Arabidopsis thaliana | 9507 | - |
cytosol | isozyme SAT-c | Arabidopsis thaliana | 5829 | - |
mitochondrion | isozyme SAT-m, matrix | Arabidopsis thaliana | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Arabidopsis thaliana | regulatory role in cysteine biosynthesis | CoA + O-acetyl-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
3 isozymes specific for subcellular compartment: SAT-c, SAT-m, SAT-p | - |
Purification (Comment) | Organism |
---|---|
partially, recombinant isozymes SAT-p, SAT-m, and SAT-c from E. coli | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | SAT-c, SAT-m, SAT-p | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Arabidopsis thaliana | CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | regulatory role in cysteine biosynthesis | Arabidopsis thaliana | CoA + O-acetyl-L-serine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000-36500, 3 recombinant isozymes, SDS-PAGE | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0074 | - |
L-cysteine | versus acetyl-CoA, isozyme SAT-c | Arabidopsis thaliana | |
0.0108 | - |
L-cysteine | versus L-serine, isozyme SAT-c | Arabidopsis thaliana |