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Literature summary for 2.3.1.29 extracted from
- Molecular cloning of the human and murine 2-amino-3-ketobutyrate coenzyme A ligase cDNAs (2000), Eur. J. Biochem., 267, 1805-1812.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| bovine partial | Mus musculus |
| bovine partial | Homo sapiens |
| bovine partial | Bos taurus |
| bovine partial | Escherichia coli |
| expressed in Escherichia coli | Mus musculus |
| expressed in Escherichia coli | Homo sapiens |
| expressed in Escherichia coli | Bos taurus |
| expressed in Escherichia coli | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| external aldimine in the crystal structure, interaction between aldimine and the side chains in the substrate binding site explain the specificity to the substrate | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | enzyme synthesized in cytoplasm | Homo sapiens | 5737 | - |
| mitochondrion | - |
Mus musculus | 5739 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
| mitochondrion | enzyme imported into mitochondrion from cytoplasm | Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
2 * 43000, SDS-PAGE | Escherichia coli |
| 80000 | - |
dynamic ligth scattering measurement | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + glycine | Mus musculus | enzyme catalyzes the second reaction step on the main metabolic degradation pathway for threonine | CoA + 2-amino-3-oxobutanoate | - |
? |  |
| acetyl-CoA + glycine | Homo sapiens | enzyme catalyzes the second reaction step on the main metabolic degradation pathway for threonine | CoA + 2-amino-3-oxobutanoate | - |
? | |
| acetyl-CoA + glycine | Bos taurus | enzyme catalyzes the second reaction step on the main metabolic degradation pathway for threonine | CoA + 2-amino-3-oxobutanoate | - |
? | |
| acetyl-CoA + glycine | Escherichia coli | enzyme catalyzes the second reaction step on the main metabolic degradation pathway for threonine | CoA + 2-amino-3-oxobutanoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Escherichia coli | P0AB77 | - |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
- |
Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + glycine = CoA + L-2-amino-3-oxobutanoate | reaction mechanism | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| liver | - |
Bos taurus | - |
| lung | - |
Mus musculus | - |
| lung | - |
Homo sapiens | - |
| pancreas | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + glycine | - |
Mus musculus | CoA + 2-amino-3-oxobutanoate | spontaneous decarboxylation to aminoacetone | r | |
| acetyl-CoA + glycine | - |
Homo sapiens | CoA + 2-amino-3-oxobutanoate | spontaneous decarboxylation to aminoacetone | r | |
| acetyl-CoA + glycine | - |
Bos taurus | CoA + 2-amino-3-oxobutanoate | spontaneous decarboxylation to aminoacetone | r | |
| acetyl-CoA + glycine | - |
Escherichia coli | CoA + 2-amino-3-oxobutanoate | spontaneous decarboxylation to aminoacetone | ? | |
| acetyl-CoA + glycine | enzyme catalyzes the second reaction step on the main metabolic degradation pathway for threonine | Mus musculus | CoA + 2-amino-3-oxobutanoate | - |
? | |
| acetyl-CoA + glycine | enzyme catalyzes the second reaction step on the main metabolic degradation pathway for threonine | Homo sapiens | CoA + 2-amino-3-oxobutanoate | - |
? | |
| acetyl-CoA + glycine | enzyme catalyzes the second reaction step on the main metabolic degradation pathway for threonine | Bos taurus | CoA + 2-amino-3-oxobutanoate | - |
? | |
| acetyl-CoA + glycine | enzyme catalyzes the second reaction step on the main metabolic degradation pathway for threonine | Escherichia coli | CoA + 2-amino-3-oxobutanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Bos taurus |
| dimer | 2 * 43000, SDS-PAGE | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Bos taurus | |
| pyridoxal 5'-phosphate | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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