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Literature summary for 2.3.1.29 extracted from
- Purification, properties, and N-terminal amino acid sequence of homogeneous Escherichia coli 2-amino-3-ketobutyrate CoA ligase, a pyridoxal phosphate-dependent enzyme (1987), J. Biol. Chem., 262, 14441-14447.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Escherichia coli |  |
| iodoacetamide | not | Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
| p-mercuribenzoate | - |
Escherichia coli | |
General Stability
| General Stability | Organism |
|---|---|
| glycerol, 10% v/v, stabilizes | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminomalonic acid | - |
Escherichia coli | |
| Aminomethylphosphonic acid | - |
Escherichia coli | |
| Aminooxyacetate | - |
Escherichia coli | |
| Ba2+ | - |
Escherichia coli | |
| Ca2+ | - |
Escherichia coli | |
| Cd2+ | strong | Escherichia coli | |
| CoA | strong | Escherichia coli | |
| Cu2+ | strong | Escherichia coli | |
| DTT | - |
Escherichia coli | |
| glutathione | - |
Escherichia coli | |
| Hg2+ | strong | Escherichia coli | |
| hydroxylamine | pyridoxal 5'-phosphate restores | Escherichia coli | |
| IAA | - |
Escherichia coli | |
| L-cysteine | - |
Escherichia coli | |
| Mn2+ | - |
Escherichia coli | |
| additional information | no inhibition by DTNB; not: PCMB, glycine methylester, glycinamide | Escherichia coli | |
| NaBH4 | - |
Escherichia coli | |
| Ni2+ | - |
Escherichia coli | |
| phenylhydrazine | - |
Escherichia coli | |
| Semicarbazide | - |
Escherichia coli | |
| Sr2+ | - |
Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.059 | - |
acetyl-CoA | - |
Escherichia coli | |
| 0.08 | - |
propionyl-CoA | - |
Escherichia coli | |
| 12 | - |
butyryl-CoA | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41690 | - |
2 * 41690, gel filtration, denaturating conditions, 2 * 41930, SDS-PAGE, 2 * 42093, calculated from amino acid composition | Escherichia coli |
| 41930 | - |
2 * 41690, gel filtration, denaturating conditions, 2 * 41930, SDS-PAGE, 2 * 42093, calculated from amino acid composition | Escherichia coli |
| 42093 | - |
2 * 41690, gel filtration, denaturating conditions, 2 * 41930, SDS-PAGE, 2 * 42093, calculated from amino acid composition | Escherichia coli |
| 84190 | - |
calculated from amino acid composition | Escherichia coli |
| 85000 | - |
gel filtration | Escherichia coli |
| 90480 | - |
sucrose density gradient centrifugation | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + glycine | Escherichia coli | involved in L-threonine catabolism, inducible | CoA + 2-amino-3-oxobutanoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K-12-mutant SBD-76, grown on L-threonine | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | no carbohydrates | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.76 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + glycine | not: valeryl-CoA, glutaryl-CoA | Escherichia coli | CoA + 2-amino-3-oxobutanoate | - |
r | |
| acetyl-CoA + glycine | specific for glycine | Escherichia coli | CoA + 2-amino-3-oxobutanoate | - |
r | |
| acetyl-CoA + glycine | succinyl-CoA | Escherichia coli | CoA + 2-amino-3-oxobutanoate | - |
r | |
| acetyl-CoA + glycine | no substrate: L-alanine | Escherichia coli | CoA + 2-amino-3-oxobutanoate | - |
r | |
| acetyl-CoA + glycine | no substrates: glycinamide, 2-aminoethanol, aminomethylphosphonic acid, aminomalonate | Escherichia coli | CoA + 2-amino-3-oxobutanoate | - |
r | |
| acetyl-CoA + glycine | no substrate: glycine methylester | Escherichia coli | CoA + 2-amino-3-oxobutanoate | - |
r | |
| acetyl-CoA + glycine | no substrates: L-serine, L-threonine, L-valine, L-leucine | Escherichia coli | CoA + 2-amino-3-oxobutanoate | - |
r | |
| acetyl-CoA + glycine | involved in L-threonine catabolism, inducible | Escherichia coli | CoA + 2-amino-3-oxobutanoate | - |
? | |
| n-butyryl-CoA + glycine | reaction at 16% the rate of acetyl-CoA | Escherichia coli | CoA + 2-amino-3-oxohexanoate | - |
? | |
| n-propionyl-CoA + glycine | reaction at 127% the rate of acetyl-CoA | Escherichia coli | CoA + 2-amino-3-oxopentanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 41690, gel filtration, denaturating conditions, 2 * 41930, SDS-PAGE, 2 * 42093, calculated from amino acid composition | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | about 30% of maximal activity at pH 6.0 and 43% at 9.0 | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
aminomalonic acid | - |
Escherichia coli | |
| 3.6 | - |
Aminomethylphosphonic acid | - |
Escherichia coli | |
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