Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.28 extracted from

  • Voloshchuk, N.; Lee, M.X.; Zhu, W.W.; Tanrikulu, I.C.; Montclare, J.K.
    Fluorinated chloramphenicol acetyltransferase thermostability and activity profile: Improved thermostability by a single-isoleucine mutant (2007), Bioorg. Med. Chem. Lett., 17, 5907-5911.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
L158I fluorinated mutant expressed in trifluoroleucine shows enhanced thermostability compared to CAT T (CAT expressed in trifluoroleucine), suggesting that trifluoroleucine at position 158 contributes to a portion of the observed loss in thermostability upon global fluorination. Relative activity: 89% (non-fluorinated mutant), 51.7% (fluorinated mutant) Escherichia coli
L208I fluorinated mutant expressed in trifluoroleucine shows loss in thermostability Escherichia coli
L821I fluorinated mutant expressed in trifluoroleucine shows loss in thermostability Escherichia coli
additional information residue-specific incorporation of T into chloramphenicol acetyltransferase (CAT) results in a loss of thermostability. Relative activity: 34.6% (fluorinated CAT) Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
relative activity: 94.9% (wild-type), 34.6% (fluorinated CAT), 89% (mutant L158I), 51.7% (fluorinated mutant L158I) Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + chloramphenicol
-
Escherichia coli CoA + chloramphenicol 3-acetate
-
?

Synonyms

Synonyms Comment Organism
CAT
-
Escherichia coli
chloramphenicol acetyltransferase
-
Escherichia coli