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Literature summary for 2.3.1.26 extracted from

  • Rogers, M.A.; Liu, J.; Kushnir, M.M.; Bryleva, E.; Rockwood, A.L.; Meikle, A.W.; Shapiro, D.; Vaisman, B.L.; Remaley, A.T.; Chang, C.C.; Chang, T.Y.
    Cellular pregnenolone esterification by acyl-CoA:cholesterol acyltransferase (2012), J. Biol. Chem., 287, 17483-17492.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.26

Activating Compound

Activating Compound Comment Organism Structure
7-oxocholesterol
-
 Homo sapiens
cholesterol acts as a strong activator and as a substrate Homo sapiens
sitosterol
-
 Homo sapiens
sitosterol weak activator Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged fusion protein in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
F12511
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 pregnenolone pH 7.4, 37°C, with cholesterol (0.32 mM). Without cholesterol, the Km for pregnenolone is too high to be measurable Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O75908
-
-
Homo sapiens P35610
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + cholesterol
-
 Homo sapiens CoA + cholesterol ester
-
 ?
pregnenolone + oleoyl-CoA without cholesterol, pregnenolone is a poor ACAT substrate, with cholesterol, the Vmax for PREG esterification increases by 100fold Homo sapiens 20-oxopregn-5-en-3beta-yl (9Z)-octadec-9-enoate + CoA
-
 ?
pregnenolone + oleoyl-CoA without cholesterol, pregnenolone is a poor ACAT substrate, with cholesterol, the Vmax for pregnenolone esterification increases by 100fold Homo sapiens 20-oxopregn-5-en-3beta-yl (9Z)-octadec-9-enoate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
ACAT1
-
 Homo sapiens
ACAT2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction mice lacking ACAT1 or ACAT2 do not have a decreased PREG ester contents in adrenals, nor do they have altered levels of the three major secreted adrenal steroids in serum Homo sapiens
malfunction mice lacking ACAT1 or ACAT2 do not have a decreased pregnenolone ester contents in adrenals, nor do they have altered levels of the three major secreted adrenal steroids in serum Homo sapiens