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Literature summary for 2.3.1.26 extracted from

  • Das, A.; Davis, M.A.; Tomoda, H.; Omura, S.; Rudel, L.L.
    Identification of the interaction site within acyl-CoA:cholesterol acyltransferase 2 for the isoform-specific inhibitor pyripyropene A (2008), J. Biol. Chem., 283, 10453-10460.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.26

Cloned(Commentary)

Cloned (Comment) Organism
all constructs, full-length, chimeras and mutants are cloned into the pCDNA3 vector, AC29 cells are transfected Chlorocebus aethiops

Protein Variants

Protein Variants Comment Organism
A1:1-123 chimeric protein A1(1-123)-A2(105-526) Chlorocebus aethiops
A1:1-385 chimeric protein A1(1-385)-A2(364-440)-A1(463-550) Chlorocebus aethiops
A1:1-71 chimeric protein A1(1-71)-A2(70-562) Chlorocebus aethiops
A2:1-227 chimeric protein A2(1-227)-A1(248-489)-A2(468-526) Chlorocebus aethiops
A2:1-249 chimeric protein A2(1-249)-A1(270-362)-A2(341-526) Chlorocebus aethiops
A2:1-363 chimeric protein A2(1-363)-A1(386-462)-A2(441-526) Chlorocebus aethiops
A2:1-428 chimeric protein A2(1-428)-A1(444-550) Chlorocebus aethiops
A2:1-440 chimeric protein A2(1-440)-A1(463-502)-A2(480-526) Chlorocebus aethiops
A2:1-479 chimeric protein A2(1-479)-A1(502-550) Chlorocebus aethiops
A2C497S A2 mutant Chlorocebus aethiops
A2I491V A2 mutant Chlorocebus aethiops
A2L480M A2 mutant Chlorocebus aethiops
A2L495F A2 mutant Chlorocebus aethiops
A2M484S A2 mutant Chlorocebus aethiops
A2Q489N A2 mutant Chlorocebus aethiops
A2Q492L A2 mutant Chlorocebus aethiops
A2R504Q A2 mutant Chlorocebus aethiops
A2S494C A2 mutant Chlorocebus aethiops
A2V493L A2 mutant Chlorocebus aethiops
Ch1 chimeric protein A2(1-428)-A1(451-462)-A2(441-526) Chlorocebus aethiops
CT504 A2(1-504) Chlorocebus aethiops

Inhibitors

Inhibitors Comment Organism Structure
pyripyropene A
-
 Chlorocebus aethiops

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00000266
-
 cis-9-octadecenoyl-CoA pyripyropene A-insensitive chimera A2:1-479 Chlorocebus aethiops
0.00000333
-
 cis-9-octadecenoyl-CoA pyripyropene A-insensitive chimera A2:1-428 Chlorocebus aethiops
0.00000553
-
 cis-9-octadecenoyl-CoA wild typeACAT2 Chlorocebus aethiops
0.00000587
-
 cis-9-octadecenoyl-CoA pyripyropene A-insensitive mutant A2S494C Chlorocebus aethiops
0.000008094
-
 cis-9-octadecenoyl-CoA pyripyropene A-insensitive mutant A2V493L Chlorocebus aethiops
0.00001218
-
 cis-9-octadecenoyl-CoA pyripyropene A-insensitive mutant A2Q492L Chlorocebus aethiops

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + cholesterol Chlorocebus aethiops
-
 CoA + cholesterol ester
-
 ?

Organism

Organism UniProt Comment Textmining
Chlorocebus aethiops
-
 African green monkey
-

Purification (Commentary)

Purification (Comment) Organism
microsomes from AC29 cells are prepared Chlorocebus aethiops

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + cholesterol
-
 Chlorocebus aethiops CoA + cholesterol ester
-
 ?
oleoyl-CoA + cholesterol
-
 Chlorocebus aethiops CoA + cholesteryl oleate
-
 ?
oleoyl-CoA + cholesterol activity assay Chlorocebus aethiops ?
-
 ?

Synonyms

Synonyms Comment Organism
A2
-
 Chlorocebus aethiops
ACAT2
-
 Chlorocebus aethiops
acyl-CoA:cholesterol acyltransferase 2
-
 Chlorocebus aethiops

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 microsomal ACAT assay Chlorocebus aethiops