Cloned (Comment) | Organism |
---|---|
presence of two gene products: ACAT1 and ACAT2, the gene product ACAT2 cloned from an African green monkey liver cDNA library. Transfection of the cloned ACAT2 cDNA into two different mammalian cell types | Platyrrhini |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CP113818 | recombinant enzyme, 80-90% inhibition at 0.0002 mM | Platyrrhini | |
PD138412 | recombinant enzyme, 80-90% inhibition at 0.003 mM | Platyrrhini |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
endoplasmic reticulum | ACAT2: rough | Platyrrhini | 5783 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
long-chain fatty acyl-CoA + cholesterol | Rattus norvegicus | - |
CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
long-chain fatty acyl-CoA + cholesterol | Platyrrhini | - |
CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
additional information | Platyrrhini | two closely related enzymes identified that catalyse the esterification of cholesterol using acyl-CoA substrates, namely ACAT1 and ACAT2, the orientation of ACAT2 in the endoplasmic reticulum membrane, in addition to its expression only in liver and intestine, suggests that this enzyme may have as a primary function, the secretion of cholesteryl esters into apolipoprotein B-containing lipoproteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Platyrrhini | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Rattus norvegicus |
glycoprotein | two potential N-glycosylation motifs at N329 and N387, only the second of which is present in the human enzyme | Platyrrhini |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | ACAT1 enzyme, high mRNA signal | Platyrrhini | - |
adrenal gland | - |
Rattus norvegicus | - |
adrenal gland | ACAT1 enzyme, highest mRNA signal | Platyrrhini | - |
heart | ACAT1 enzyme, high mRNA signal | Platyrrhini | - |
hepatocyte | - |
Rattus norvegicus | - |
hepatocyte | two different forms of the enzyme are present, ACAT1 and ACAT2 | Platyrrhini | - |
intestine | - |
Rattus norvegicus | - |
intestine | expression of ACAT2 mRNA | Platyrrhini | - |
kidney | ACAT1 enzyme, high mRNA signal | Platyrrhini | - |
liver | - |
Rattus norvegicus | - |
liver | expression of ACAT2 mRNA. ACAT1 enzyme: high mRNA signal | Platyrrhini | - |
pancreas | ACAT1 enzyme, high mRNA signal | Platyrrhini | - |
trachea | ACAT1 enzyme, high mRNA signal | Platyrrhini | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0012 | - |
recombinant enzyme ACAT1, vector: pcDNA3+, cell type: AC29 | Platyrrhini |
0.0017 | - |
liver microsomes | Rattus norvegicus |
0.0019 | - |
recombinant enzyme ACAT2, vector: pCMV5, cell type: AC29 | Platyrrhini |
0.0022 | - |
recombinant enzyme ACAT2, vector: pcDNA3+, cell type: AC29 | Platyrrhini |
0.0038 | - |
recombinant enzyme ACAT2, vector: pcDNA3+, cell type: COS | Platyrrhini |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
long-chain fatty acyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
long-chain fatty acyl-CoA + cholesterol | - |
Platyrrhini | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
additional information | two closely related enzymes identified that catalyse the esterification of cholesterol using acyl-CoA substrates, namely ACAT1 and ACAT2, the orientation of ACAT2 in the endoplasmic reticulum membrane, in addition to its expression only in liver and intestine, suggests that this enzyme may have as a primary function, the secretion of cholesteryl esters into apolipoprotein B-containing lipoproteins | Platyrrhini | ? | - |
? |