Activating Compound | Comment | Organism | Structure |
---|---|---|---|
25-hydroxycholesterol | activates | Homo sapiens | |
6-ketocholestanol | added at high concentration causes modest increase in enzyme activity | Homo sapiens | |
7-ketocholesterol | added at high concentration causes modest increase in enzyme activity | Homo sapiens | |
cholesterol | serves as an enzyme activator in vitro, in addition to its role as an enzyme substrate | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in insect SF9 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CI-976 | inhibits | Homo sapiens | |
DuP 128 | inhibits | Homo sapiens | |
octimibate | inhibits | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
solubilization of the cell extracts with deoxycholate followed by dilution into preformed liposomes | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activation of the enzyme by adding 25-hydroxycholesterol to intact cells or to cell-free extracts | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleoyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesteryl oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | SDS-PAGE: detection of two protein bands with apparent sizes at 50 and 56 kDa, immunoblots: existence of 100 and 200 kDa bands, clarification of these observations needed | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme activity expressed in Sf9 insect cells exhibits heat stability comparable of that of human enzyme, its heat stability is significantly greater than that of hamster enzyme | Homo sapiens |