Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.26 extracted from

  • Cheng, D.; Chang C.C.Y.; Qu, X.; Chang, T.
    Activation of acyl-coenzyme A:cholesterol acyltransferase by cholesterol or by oxysterol in a cell-free system (1995), J. Biol. Chem., 270, 685-695.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
25-hydroxycholesterol activates Homo sapiens
6-ketocholestanol added at high concentration causes modest increase in enzyme activity Homo sapiens
7-ketocholesterol added at high concentration causes modest increase in enzyme activity Homo sapiens
cholesterol serves as an enzyme activator in vitro, in addition to its role as an enzyme substrate Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in insect SF9 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CI-976 inhibits Homo sapiens
DuP 128 inhibits Homo sapiens
octimibate inhibits Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Homo sapiens 5783
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
solubilization of the cell extracts with deoxycholate followed by dilution into preformed liposomes Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activation of the enzyme by adding 25-hydroxycholesterol to intact cells or to cell-free extracts Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oleoyl-CoA + cholesterol
-
Homo sapiens CoA + cholesteryl oleate
-
?

Subunits

Subunits Comment Organism
More SDS-PAGE: detection of two protein bands with apparent sizes at 50 and 56 kDa, immunoblots: existence of 100 and 200 kDa bands, clarification of these observations needed Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
enzyme activity expressed in Sf9 insect cells exhibits heat stability comparable of that of human enzyme, its heat stability is significantly greater than that of hamster enzyme Homo sapiens