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Literature summary for 2.3.1.26 extracted from
- Acyl coenzyme A:cholesterol O-acyltransferase (1983), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 16, 523-539.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cholesterol | - |
Cricetulus griseus |  |
| cholesterol | exogenous, added as phosphatidylcholine liposome or in acetone solution, stimulates | Platyrrhini | |
| cholesterol | exogenous, delivered as a Triton WR-1339 detergent dispersion, increases activity | Rattus norvegicus | |
| cholesterol | exogenous, not stimulatory | Homo sapiens | |
| cholesterol | exogenous, not stimulatory | Sus scrofa | |
| cholesterol | exogenous, not stimulatory | Oryctolagus cuniculus | |
| additional information | regulation by sterol | Cricetulus griseus | |
| additional information | regulation by sterol | Cavia porcellus | |
| additional information | regulation by sterol | Mus musculus | |
| additional information | regulation by sterol | Homo sapiens | |
| additional information | regulation by sterol | Rattus norvegicus | |
| additional information | regulation by sterol | Sus scrofa | |
| additional information | regulation by sterol | Oryctolagus cuniculus | |
| additional information | regulation by sterol | Platyrrhini | |
| additional information | regulation by sterol | Columba sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 20alpha-hydroxypregn-4-en-3-one | - |
Oryctolagus cuniculus | |
| Detergents | ionic and non-ionic | Cavia porcellus | |
| Detergents | ionic and non-ionic | Columba sp. | |
| Detergents | ionic and non-ionic | Cricetulus griseus | |
| Detergents | ionic and non-ionic | Homo sapiens | |
| Detergents | ionic and non-ionic | Mus musculus | |
| Detergents | ionic and non-ionic | Oryctolagus cuniculus | |
| Detergents | ionic and non-ionic | Platyrrhini | |
| Detergents | ionic and non-ionic | Rattus norvegicus | |
| Detergents | ionic and non-ionic | Sus scrofa | |
| lidocaine | - |
Oryctolagus cuniculus | |
| lidocaine | - |
Rattus norvegicus | |
| oleoyl-CoA | inhibits at high concentrations, the inhibition is prevented by serum albumin | Rattus norvegicus | |
| phosphatidylinositol | - |
Sus scrofa | |
| phosphatidylserine | not inhibitory | Rattus norvegicus | |
| phosphatidylserine | - |
Sus scrofa | |
| Polyoxyethylated cholesterol | - |
Homo sapiens | |
| progesterone | - |
Homo sapiens | |
| progesterone | - |
Oryctolagus cuniculus | |
| progesterone | - |
Rattus norvegicus | |
| SC-31769 | 20-oxa analog of 7-ketosterol | Homo sapiens | |
| SC-31769 | 20-oxa analog of 7-ketosterol | Rattus norvegicus | |
| sphingomyelin | - |
Mus musculus | |
| Tetracaine | - |
Oryctolagus cuniculus | |
| Tetracaine | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
oleoyl-CoA | - |
Rattus norvegicus | |
| 0.004 | - |
oleoyl-CoA | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Cricetulus griseus | 5783 | - |
| endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| endoplasmic reticulum | - |
Platyrrhini | 5783 | - |
| endoplasmic reticulum | rough, membranes | Rattus norvegicus | 5783 | - |
| microsome | - |
Mus musculus | - |
- |
| microsome | - |
Homo sapiens | - |
- |
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Oryctolagus cuniculus | - |
- |
| microsome | cytoplasmic surface of | Sus scrofa | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| long-chain fatty acyl-CoA + cholesterol | Cricetulus griseus | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Cavia porcellus | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Mus musculus | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Homo sapiens | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Rattus norvegicus | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Sus scrofa | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Oryctolagus cuniculus | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Platyrrhini | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Columba sp. | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Cricetulus griseus | responsible for cellular synthesis of cholesterol esters in various cell types | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Cavia porcellus | responsible for cellular synthesis of cholesterol esters in various cell types | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Mus musculus | responsible for cellular synthesis of cholesterol esters in various cell types | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Homo sapiens | responsible for cellular synthesis of cholesterol esters in various cell types | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Rattus norvegicus | responsible for cellular synthesis of cholesterol esters in various cell types | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Sus scrofa | responsible for cellular synthesis of cholesterol esters in various cell types | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Oryctolagus cuniculus | responsible for cellular synthesis of cholesterol esters in various cell types | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Platyrrhini | responsible for cellular synthesis of cholesterol esters in various cell types | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | Columba sp. | responsible for cellular synthesis of cholesterol esters in various cell types | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
| Columba sp. | - |
pigeon | - |
| Cricetulus griseus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Platyrrhini | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using ammonium acetate fractionation and Sepharose 4B column chromatography | Sus scrofa |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| solubilization of enzyme activity by deoxycholate, reconstitution of the solubilized activity into liposome of known cholesterol and phospholipid content by employing a cholate dialysis procedure | Cricetulus griseus |
| solubilization of enzyme activity by deoxycholate, reconstitution of the solubilized activity into liposome of known cholesterol and phospholipid content by employing a cholate dialysis procedure | Sus scrofa |
| solubilization of enzyme activity from Ehrlich ascites cells, the solubilized activity can be reconstituted as a liposome complex after the detergent is removed | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Mus musculus | - |
| adrenal gland | - |
Homo sapiens | - |
| adrenal gland | - |
Rattus norvegicus | - |
| adrenal gland | - |
Oryctolagus cuniculus | - |
| adrenal gland | - |
Platyrrhini | - |
| artery | - |
Oryctolagus cuniculus | - |
| artery | - |
Platyrrhini | - |
| artery | - |
Columba sp. | - |
| CHO cell | CHO cells, cultured cells | Cricetulus griseus | - |
| Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
| fibroblast | - |
Homo sapiens | - |
| hepatocyte | - |
Rattus norvegicus | - |
| hepatocyte | - |
Platyrrhini | - |
| hepatoma cell | - |
Rattus norvegicus | - |
| intestine | - |
Mus musculus | - |
| intestine | - |
Homo sapiens | - |
| intestine | - |
Rattus norvegicus | - |
| intestine | - |
Oryctolagus cuniculus | - |
| intestine | - |
Platyrrhini | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Sus scrofa | - |
| liver | - |
Oryctolagus cuniculus | - |
| liver | - |
Platyrrhini | - |
| liver | - |
Columba sp. | - |
| peritoneal macrophage | - |
Cricetulus griseus | - |
| peritoneal macrophage | - |
Cavia porcellus | - |
| peritoneal macrophage | - |
Mus musculus | - |
| peritoneal macrophage | - |
Rattus norvegicus | - |
| peritoneal macrophage | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay method | Cricetulus griseus |
| additional information | - |
assay method | Cavia porcellus |
| additional information | - |
assay method | Mus musculus |
| additional information | - |
assay method | Homo sapiens |
| additional information | - |
assay method | Rattus norvegicus |
| additional information | - |
assay method | Sus scrofa |
| additional information | - |
assay method | Oryctolagus cuniculus |
| additional information | - |
assay method | Platyrrhini |
| additional information | - |
assay method | Columba sp. |
| 0.00008 | - |
solubilized enzyme | Sus scrofa |
| 0.015 | - |
partially purified enzyme | Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, liver microsomal enzyme, stable for several months | Rattus norvegicus |
| -70°C, liver microsomal enzyme, stable for several months | Sus scrofa |
| -70°C, liver microsomal enzyme, stable for several months | Platyrrhini |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + cholesterol | - |
Cricetulus griseus | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Cavia porcellus | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Mus musculus | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Sus scrofa | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Oryctolagus cuniculus | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Platyrrhini | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Columba sp. | CoA + cholesterol ester | - |
? | |
| linoleoyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl linoleate | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | Cricetulus griseus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | Cavia porcellus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | Mus musculus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | Homo sapiens | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | Rattus norvegicus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | Sus scrofa | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | Oryctolagus cuniculus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | Platyrrhini | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | may play an important role in regulation of the accumulation of cholesterol esters within smooth muscle cells of the artery wall during atherogenesis and in synthesis of cholesterol esters during hepatic very low-density lipoprotein synthesis and secretion | Columba sp. | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | responsible for cellular synthesis of cholesterol esters in various cell types | Cricetulus griseus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | responsible for cellular synthesis of cholesterol esters in various cell types | Cavia porcellus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | responsible for cellular synthesis of cholesterol esters in various cell types | Mus musculus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | responsible for cellular synthesis of cholesterol esters in various cell types | Homo sapiens | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | responsible for cellular synthesis of cholesterol esters in various cell types | Rattus norvegicus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | responsible for cellular synthesis of cholesterol esters in various cell types | Sus scrofa | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | responsible for cellular synthesis of cholesterol esters in various cell types | Oryctolagus cuniculus | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | responsible for cellular synthesis of cholesterol esters in various cell types | Platyrrhini | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| long-chain fatty acyl-CoA + cholesterol | responsible for cellular synthesis of cholesterol esters in various cell types | Columba sp. | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| additional information | - |
Homo sapiens | ? | - |
? | |
| additional information | campesterol is a poor substrate, indicating the alkyl side chain plays an important role in the enzyme reaction | Rattus norvegicus | ? | - |
? | |
| additional information | sitosterol, stigmasterol, ergosterol, lanosterol, cycloartenol are not substrates | Rattus norvegicus | ? | - |
? | |
| additional information | 3-epicholesterol is not a substrate, indicating a requirement for a 3beta-hydroxyl group | Rattus norvegicus | ? | - |
? | |
| oleoyl-CoA + 4alpha-methylcholest-7-en-3beta-ol | - |
Rattus norvegicus | CoA + 4alpha-methylcholest-7-en 3-oleate | - |
? | |
| oleoyl-CoA + campesterol | campesterol is a poor substrate | Rattus norvegicus | CoA + campesteryl oleate | - |
? | |
| oleoyl-CoA + cholest-4-en-3beta-ol | low activity | Rattus norvegicus | CoA + cholest-4-en 3-oleate | - |
? | |
| oleoyl-CoA + cholestanol | cholestanol is esterified by the enzyme at almost the same rate as cholesterol | Rattus norvegicus | CoA + cholestanyl oleate | - |
? | |
| oleoyl-CoA + cholesterol | - |
Mus musculus | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + cholesterol | - |
Sus scrofa | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + cholesterol | - |
Oryctolagus cuniculus | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + cholesterol | - |
Platyrrhini | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + cholesterol | highest activity | Rattus norvegicus | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + desmosterol | - |
Rattus norvegicus | CoA + desmosteryl oleate | - |
? | |
| oleoyl-CoA + lathosterol | - |
Rattus norvegicus | CoA + lathosteryl oleate | - |
? | |
| palmitoyl-CoA + cholesterol | - |
Mus musculus | CoA + cholesteryl palmitate | - |
? | |
| palmitoyl-CoA + cholesterol | approximately 50% of the activity with oleoyl-CoA | Rattus norvegicus | CoA + cholesteryl palmitate | - |
? | |
| stearoyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl stearate | - |
? | |
| stearyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl stearate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | assay at | Cricetulus griseus |
| 30 | 37 | assay at | Cavia porcellus |
| 30 | 37 | assay at | Mus musculus |
| 30 | 37 | assay at | Homo sapiens |
| 30 | 37 | assay at | Rattus norvegicus |
| 30 | 37 | assay at | Sus scrofa |
| 30 | 37 | assay at | Oryctolagus cuniculus |
| 30 | 37 | assay at | Platyrrhini |
| 30 | 37 | assay at | Columba sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
liver enzyme, 10 min, activity destroyed | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
progesterone | - |
Oryctolagus cuniculus | |
| 0.05 | - |
20alpha-hydroxypregn-4-en-3-one | - |
Oryctolagus cuniculus | |
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