Activating Compound | Comment | Organism | Structure |
---|---|---|---|
albumin | - |
Homo sapiens | |
albumin | required in the assay medium to prevent the high concentration of oleoly-CoA from inhibiting the enzyme presumably by disrupting the microsomal membrane | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(Z)-17(20)-dehydrocholesterol | inhibits esterification of endogenous cholesterol | Rattus norvegicus | |
20-Methylcholesterol | inhibits esterification of endogenous cholesterol | Rattus norvegicus | |
5alpha-dihydroprogesterone | inhibits esterification of endogenous cholesterol | Homo sapiens | |
acetone | - |
Rattus norvegicus | |
acetone | about 40% inhibition at a final concentration of 1% v/v | Saccharomyces cerevisiae | |
androsterone | inhibits esterification of endogenous cholesterol | Rattus norvegicus | |
butanol | - |
Rattus norvegicus | |
butanol | about 40% inhibition at a final concentration of 1% v/v | Saccharomyces cerevisiae | |
Cycloartenol | inhibits esterification of exogenous cholesterol | Rattus norvegicus | |
deoxycorticosterone | inhibits esterification of endogenous cholesterol | Homo sapiens | |
deoxycorticosterone | - |
Rattus norvegicus | |
Detergents | - |
Homo sapiens | |
Detergents | above their critical micelle concentration, e.g. cholate, deoxycholate, cetyltriethylammonium bromide, Triton X-100, Tween 20, Tween 80, Nonidet P40 | Rattus norvegicus | |
Detergents | - |
Sus scrofa | |
Digitonin | - |
Rattus norvegicus | |
ergosterol | inhibits esterification of exogenous cholesterol | Rattus norvegicus | |
ethanol | - |
Rattus norvegicus | |
ethanol | about 40% inhibition at a final concentration of 1% v/v | Saccharomyces cerevisiae | |
Filipin | - |
Rattus norvegicus | |
lanosterol | inhibits esterification of exogenous cholesterol | Helicoverpa zea | |
lanosterol | inhibits esterification of exogenous cholesterol | Rattus norvegicus | |
oleoyl-CoA | inhibits at high concentrations, the inhibition is prevented by serum albumin | Rattus norvegicus | |
organic solvents | inhibit by perturbing the membrane structure | Rattus norvegicus | |
Pregn-5-en-3beta-ol | inhibits esterification of endogenous cholesterol | Rattus norvegicus | |
pregnenolone | inhibits esterification of endogenous cholesterol | Homo sapiens | |
progesterone | inhibits esterification of endogenous cholesterol reversibly | Homo sapiens | |
progesterone | inhibits esterification of endogenous cholesterol reversibly | Rattus norvegicus | |
SC-31769 | 20-oxa analog of 7-ketosterol | Homo sapiens | |
SC-31769 | 20-oxa analog of 7-ketosterol | Rattus norvegicus | |
sitosterol | inhibits esterification of exogenous cholesterol | Rattus norvegicus | |
stanolone | inhibits esterification of endogenous cholesterol | Rattus norvegicus | |
stigmasterol | inhibits esterification of exogenous cholesterol | Rattus norvegicus | |
testosterone | inhibits esterification of endogenous cholesterol | Homo sapiens | |
testosterone | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens | |
additional information | - |
additional information | use of kinetic parameters such as Km is of limited value due to the physical properties of oleoyl-CoA in solution and the addition of serum albumin to the assay | Rattus norvegicus | |
0.055 | - |
oleoyl-CoA | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
microsome | - |
Homo sapiens | - |
- |
microsome | - |
Rattus norvegicus | - |
- |
microsome | - |
Sus scrofa | - |
- |
microsome | - |
Saccharomyces cerevisiae | - |
- |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | the use of organic solvents or strong detergents for the suspension of cholesterol must be avoided because of their affect on the enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicoverpa zea | - |
- |
- |
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Sus scrofa | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
the enzyme must be reconstituted into an artificial membrane for optimal activity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
peritoneal macrophage | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
additional information | - |
assay method | Homo sapiens |
additional information | - |
assay method | Rattus norvegicus |
additional information | - |
assay method | Sus scrofa |
additional information | - |
assay method | Helicoverpa zea |
Storage Stability | Organism |
---|---|
-80°C, stable for several weeks | Rattus norvegicus |
-80°C, stable for several weeks | Saccharomyces cerevisiae |
-80°C, stable for several weeks | Helicoverpa zea |
ovary, labile to freezing | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesterol ester | - |
? | |
acyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesterol ester | - |
? | |
acyl-CoA + cholesterol | - |
Sus scrofa | CoA + cholesterol ester | - |
? | |
acyl-CoA + cholesterol | - |
Saccharomyces cerevisiae | CoA + cholesterol ester | - |
? | |
acyl-CoA + cholesterol | - |
Helicoverpa zea | CoA + cholesterol ester | - |
? | |
linoleoyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl linoleate | - |
? | |
additional information | - |
Homo sapiens | ? | - |
? | |
additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
additional information | a part of the substrate selectivity may be due to the presence of acyl-CoA hydrolase in the microsomal preparations. For this enzyme, the preferred substrate is palmitoyl-CoA which is hydrolysed about 8times faster than oleoyl-CoA | Rattus norvegicus | ? | - |
? | |
additional information | investigations into the sterol specificity of the enzyme | Rattus norvegicus | ? | - |
? | |
oleoyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesteryl oleate | - |
? | |
oleoyl-CoA + cholesterol | - |
Sus scrofa | CoA + cholesteryl oleate | - |
? | |
oleoyl-CoA + cholesterol | highest activity | Rattus norvegicus | CoA + cholesteryl oleate | - |
? | |
oleoyl-CoA + cholesterol | highest activity | Saccharomyces cerevisiae | CoA + cholesteryl oleate | - |
? | |
oleoyl-CoA + ergosterol | - |
Homo sapiens | CoA + ergosteryl oleate | - |
? | |
oleoyl-CoA + ergosterol | - |
Saccharomyces cerevisiae | CoA + ergosteryl oleate | - |
? | |
palmitoyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl palmitate | - |
? | |
palmitoyl-CoA + cholesterol | - |
Saccharomyces cerevisiae | CoA + cholesteryl palmitate | - |
? | |
stearoyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl stearate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Sus scrofa |
37 | - |
assay at | Helicoverpa zea |