Cloned (Comment) | Organism |
---|---|
gene PORCN, quantitative PCR enzyme expression analysis | Mus musculus |
gene PORCN, quantitative RT-PCR enzyme expression analysis. PORCN splice variants differ globally in signaling activity in HT-1080 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H341A | site-directed mutagenesis, catalytic active site mutant, Wnt signaling inactive mutant | Mus musculus |
additional information | PORCN is deleted by adenoviral Cre-GFP excision in immortalized MEF cells, PORCN null cells cannot activate WNT3A | Mus musculus |
additional information | zinc finger nuclease-mediated PORCN gene deletion in HT1080 cells. Mutation or skipping of PORCN exon 9 creates cells null for PORCN catalytic activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | integral membrane protein, enzyme structure and topology model with cytoplasmic and endoplasmic reticulum luman parts, overview | Homo sapiens | 5789 | - |
endoplasmic reticulum membrane | integral membrane protein, enzyme structure and topology model with cytoplasmic and endoplasmic reticulum luman parts, overview | Mus musculus | 5789 | - |
additional information | the enzyme is predicted to palmitoylate Wnt either co-translationally or post-translationally, so that in either case, the catalytic site is likely to be in the membrane or in the lumen of the endoplasmic reticulum | Homo sapiens | - |
- |
additional information | the enzyme is predicted to palmitoylate Wnt either co-translationally or post-translationally, so that in either case, the catalytic site is likely to be in the membrane or in the lumen of the endoplasmic reticulum | Mus musculus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine | Homo sapiens | the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a | CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine | Mus musculus | the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a | CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | Homo sapiens | - |
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | Mus musculus | - |
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
additional information | Homo sapiens | PORCN splice variants differ globally in signaling activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9H237 | gene PORCN | - |
Mus musculus | Q9JJJ7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Mus musculus | - |
fibroblast | - |
Mus musculus | - |
fibrosarcoma cell | - |
Homo sapiens | - |
HT-1080 cell | - |
Homo sapiens | - |
MEF cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine | the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a | Homo sapiens | CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine | the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a | Mus musculus | CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | - |
Homo sapiens | CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine | - |
Mus musculus | CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine | - |
? | |
additional information | PORCN splice variants differ globally in signaling activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PORCN | - |
Homo sapiens |
PORCN | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | gene deletion causes embryonic lethality in mice. PORCN null cells cannot activate WNT3A, PORCN null cells do not secrete WNT3A | Mus musculus |
malfunction | mutations in gene PORCN are associated with focal dermal hypoplasia. PORCN null cells are completely incapable of autocrine Wnt signaling | Homo sapiens |
additional information | structure-function analysis, overview | Homo sapiens |
additional information | structure-function analysis, overview | Mus musculus |
physiological function | the enzyme is a membrane-bound O-acyltransferase and is a key non-redundant node for the regulation of global Wnt signaling, essential regulatory role of PORCN in shaping Wnt signaling gradients. All Wnt activity absolutely requires posttranslational modification by the O-acyltransferase PORCN, whose abundance or activity modulates signaling tightly and over a large dynamic range, the enzyme activity is a key modulator of all Wnt ligand activity | Homo sapiens |
physiological function | the enzyme is a membrane-bound O-acyltransferase and is a key non-redundant node for the regulation of global Wnt signaling, essential regulatory role of PORCN in shaping Wnt signaling gradients. All Wnt activity absolutely requires posttranslational modification by the O-acyltransferase PORCN, whose abundance or activity modulates signaling tightly and over a large dynamic range, the enzyme activity is a key modulator of all Wnt ligand activity | Mus musculus |