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Literature summary for 2.3.1.249 extracted from

  • Luo, J.; Fuell, C.; Parr, A.; Hill, L.; Bailey, P.; Elliott, K.; Fairhurst, S.A.; Martin, C.; Michael, A.J.
    A novel polyamine acyltransferase responsible for the accumulation of spermidine conjugates in Arabidopsis seed (2009), Plant Cell, 21, 318-333.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene At2g25150, quantitative real-time RT-PCR expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 Rosetta (DE3) pLysS Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0106
-
4-coumaroyl-CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
0.0527
-
spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 4-coumaroyl-CoA + spermidine Arabidopsis thaliana the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?
2 4-coumaroyl-CoA + spermidine Arabidopsis thaliana Col-0 the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8GYW8 gene At2g25150
-
Arabidopsis thaliana Col-0 Q8GYW8 gene At2g25150
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 Rosetta (DE3) pLysS by nickel affinity chromatography and desalting gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
root the enzyme is expressed specifically in roots Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 4-coumaroyl-CoA + spermidine the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo Arabidopsis thaliana 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?
2 4-coumaroyl-CoA + spermidine the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo and is absolutely specific for 4-coumaroyl-CoA and spermidine Arabidopsis thaliana 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?
2 4-coumaroyl-CoA + spermidine the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo Arabidopsis thaliana Col-0 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?
2 4-coumaroyl-CoA + spermidine the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo and is absolutely specific for 4-coumaroyl-CoA and spermidine Arabidopsis thaliana Col-0 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?
additional information no activity with acyl donors sinapoyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors putrescine, cadaverine, spermine, agmatine, and tyramine Arabidopsis thaliana ?
-
?
additional information no activity with acyl donors sinapoyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors putrescine, cadaverine, spermine, agmatine, and tyramine Arabidopsis thaliana Col-0 ?
-
?

Synonyms

Synonyms Comment Organism
BAHD acyl transferase
-
Arabidopsis thaliana
SCT
-
Arabidopsis thaliana
spermidine coumaroyl CoA acyltransferase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
3.4
-
4-coumaroyl-CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Arabidopsis thaliana

General Information

General Information Comment Organism
evolution the enzyme is a member of the BAHD acyltransferase family Arabidopsis thaliana