Application | Comment | Organism |
---|---|---|
synthesis | versatile method for generating and identifying functional chimeric PKS enzymes for synthesizing custom macrolactones and macrolides. PKS genes from the pikromycin and erythromycin pathways are hybridized in Saccharomyces cerevisiae to generate hybrid libraries. Streptomyces venezuelae strains that expressed active chimeric enzymes with new functionality are capable of producing engineered macrolactones | Streptomyces venezuelae |
Cloned (Comment) | Organism |
---|---|
expression in Streptomyces venezuelae | Streptomyces venezuelae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces venezuelae | - |
- |
- |
Streptomyces venezuelae ATCC 15439 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylmalonyl-N-acetylcysteamine + S-phenyl (2S,4R,6E,8R,9R)-9-hydroxy-2,4,8-trimethyl-5-oxoundec-6-enethioate | - |
Streptomyces venezuelae | 3-dihydro-narbonolide + 10-deoxymethynolide + CoA + CO2 + NADP+ + H2O | products formed by chimeric polykeitde synthase construcuts | ? | |
methylmalonyl-N-acetylcysteamine + S-phenyl (2S,4R,6E,8R,9R)-9-hydroxy-2,4,8-trimethyl-5-oxoundec-6-enethioate | - |
Streptomyces venezuelae ATCC 15439 | 3-dihydro-narbonolide + 10-deoxymethynolide + CoA + CO2 + NADP+ + H2O | products formed by chimeric polykeitde synthase construcuts | ? |
Synonyms | Comment | Organism |
---|---|---|
PikAIII | - |
Streptomyces venezuelae |
PikAIV | - |
Streptomyces venezuelae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme utilizes an oxidized quinone as co-factor | Streptomyces venezuelae |