Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BJ5183, and transient expression in HEK293A cells, HT29 cells, and SW620 cells from an adenovirus vector | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
H212A | site-directed mutagenesis | Mus musculus |
additional information | enzyme knockout by shRNA | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no enzyme inhibition by curcumin | Mus musculus | |
SP600125 | - |
Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | the enzyme catalyzes the synthesis of significantly more 17C16- and 17C24:1-ceramides and significantly less 17C22:0-, 17C24:0, and 17C26:0-ceramides in vivo, analysis of sphingosine and ceramidases in different cells, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8C172 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Mus musculus | - |
breast | - |
Mus musculus | - |
colon | - |
Mus musculus | - |
HCT-116 cell | - |
Mus musculus | - |
head and neck squamous carcinoma cell | - |
Mus musculus | - |
intestine | high expression level | Mus musculus | - |
lung | - |
Mus musculus | - |
MCF-7 cell | - |
Mus musculus | - |
SW-480 cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes the synthesis of significantly more 17C16- and 17C24:1-ceramides and significantly less 17C22:0-, 17C24:0, and 17C26:0-ceramides in vivo, analysis of sphingosine and ceramidases in different cells, overview | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ceramide synthase 6 | - |
Mus musculus |
CerS6 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | decreased enzyme expression induces endoplasmic reticulum stress and apoptosis in head and neck squamous carcinoma cells but not in A-549, MCF-7, or SW-480 cells, which are derived from lung, breast, and colon cancer, respectively, detailed overview | Mus musculus |
metabolism | due to the highly dynamic nature of sphingolipid metabolism, alterations in the expression of CerS6 may have impacts beyond increased generation of C16-ceramide | Mus musculus |
physiological function | activity of CerS in the salvage pathway in which sphingosine is recycled into ceramides. Upregulation of CerS6 expression increases C16-ceramide generation at the expense of very long chain ceramides. Increased expression of acid ceramidase in response to CerS6 expression occurs via a JNK-AP1-dependent mechanism and is important for survival. Enzyme CerS6 may transcriptionally activate the expression of acid ceramidase (ASAH1) | Mus musculus |