Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces sahachiroi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sahachiroi | B4XYB8 | - |
- |
Streptomyces sahachiroi NRRL 2485 | B4XYB8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+ | - |
Streptomyces sahachiroi | 5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+ | incubation of substrates with isoform AziB alone leads to a truncated single-ring product, 2-methylbenzoic acid. Presence of thioesterase AziG acting as a chain elongation and cyclization domain is required for additional two rounds of chain extension to form the expected product, 5-methyl-1-naphthoate. None of the other thioesterase domains (AziA6, AziA7, AziA8) are capable of catalyzing the reaction | ? | |
acetyl-CoA + 5 malonyl-CoA + 3 NADPH + 3 H+ | - |
Streptomyces sahachiroi NRRL 2485 | 5-methyl-1-naphthoate + 6 CoA + 5 CO2 + 4 H2O + 3 NADP+ | incubation of substrates with isoform AziB alone leads to a truncated single-ring product, 2-methylbenzoic acid. Presence of thioesterase AziG acting as a chain elongation and cyclization domain is required for additional two rounds of chain extension to form the expected product, 5-methyl-1-naphthoate. None of the other thioesterase domains (AziA6, AziA7, AziA8) are capable of catalyzing the reaction | ? |
Synonyms | Comment | Organism |
---|---|---|
AziB | - |
Streptomyces sahachiroi |