Cloned (Comment) | Organism |
---|---|
overexpression of the tcmKL genes together in Streptomyces lividans to obtain the KS subunits | Streptomyces glaucescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10 malonyl-CoA | Streptomyces glaucescens | the multi-domain polyketide synthase involved in the synthesis of tetracenomycin | tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces glaucescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of the TcmK/TcmL alphabeta complex | Streptomyces glaucescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10 malonyl-CoA | the multi-domain polyketide synthase involved in the synthesis of tetracenomycin | Streptomyces glaucescens | tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O | - |
? | |
10 malonyl-CoA | the multi-domain polyketide synthase involves a ketosynthase complex (TcmKL), an acyl carrier protein (TcmM), a malonyl CoA:ACP acyltransferase (MAT), and a cyclase (TcmN). A malonyl-CoA molecule is initially bound to the acyl carrier protein and decarboxylated to form an acetyl starter unit. Additional two-carbon units are added from nine more malonyl-CoA molecules | Streptomyces glaucescens | tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O | tetracenomycin F2 i.e. 4-(3-acetyl-4,5,7,10-tetrahydroxyanthracen-2-yl)-3-oxobutanoic acid | ? |
Subunits | Comment | Organism |
---|---|---|
More | the TcmKL KS alphabeta ketosynthase complex, an acyl carrier protein TcmM ACP, TcmN cyclase, and FabD malonyl CoA:ACP acyltransferase proteins constitute the minimal requirements for a functional tetracenomycin polyketide synthase in vitro | Streptomyces glaucescens |
Synonyms | Comment | Organism |
---|---|---|
TCM F2 | - |
Streptomyces glaucescens |
TCM PKS | - |
Streptomyces glaucescens |
type II polyketide synthase | - |
Streptomyces glaucescens |
General Information | Comment | Organism |
---|---|---|
metabolism | the multi-domain polyketide synthase involved in the synthesis of tetracenomycin | Streptomyces glaucescens |