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Literature summary for 2.3.1.233 extracted from

  • Austin, M.B.; Izumikawa, M.; Bowman, M.E.; Udwary, D.W.; Ferrer, J.L.; Moore, B.S.; Noel, J.P.
    Crystal structure of a bacterial type III polyketide synthase and enzymatic control of reactive polyketide intermediates (2004), J. Biol. Chem., 279, 45162-45174.
    View publication on PubMed
Search for more literature for 2.3.1.233

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 14% (w/v) PEG 8000, 200 mM MgCl2, 100 mM Na-MOPSO buffer (pH 7.0), 5 mM dithiothreitol, and 3% (w/v) sucrose Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
C106S inactive Streptomyces coelicolor
C171S the mutant shows about 4fold reduced catalytic efficiency compared to the wild type enzyme Streptomyces coelicolor
C184S the mutant shows about 2.5fold increased catalytic efficiency compared to the wild type enzyme Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 malonyl-CoA mutant enzyme C184S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
0.0023
-
 malonyl-CoA wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
0.0085
-
 malonyl-CoA mutant enzyme C171S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5 malonyl-CoA Streptomyces coelicolor
-
 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5 malonyl-CoA
-
 Streptomyces coelicolor 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
1,3,6,8-tetrahydroxynaphthalene synthase
-
 Streptomyces coelicolor
PKS
-
 Streptomyces coelicolor
THNS
-
 Streptomyces coelicolor
type III polyketide synthase
-
 Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
45.7
-
 malonyl-CoA wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
46.5
-
 malonyl-CoA mutant enzyme C171S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
79.2
-
 malonyl-CoA mutant enzyme C184S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.55
-
 malonyl-CoA mutant enzyme C171S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
1.97
-
 malonyl-CoA wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor
5.53
-
 malonyl-CoA mutant enzyme C184S, in 100 mM Tris-HCl buffer (pH 7.5), at 22°C Streptomyces coelicolor