Literature summary for 2.3.1.23 extracted from

Pan, X.; Chen, G.; Kazachkov, M.; Greer, M.S.; Caldo, K.M.; Zou, J.; Weselake, R.J.
In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacylglycerol acyltransferase (2015), J. Biol. Chem., 290, 18068-18078.

Cloned(Commentary)

Cloned (Comment)	Organism
two genes LPCAT, LPCAT1 and LPCAT2, containing eight exons and seven introns, respectively, DNA and amino acid seqquence determination and analysis, co-expression of flax genes LPCAT1 and diacylglycerol acyltransferase DGAT1-1 in the yeast quintuple mutant strain H1246 DELTAale1 significantly increasing 18-carbon polyunsaturated fatty acids in triacylglycerol with a concomitant decrease of 18-carbon polyunsaturated fatty acids in phospholipid. The specific activity of flax LPCAT1-catalyzed forward reaction is about 1000 times higher than that of flax DGAT1-catalyzed reaction when the expressed recombinant enzymes are assessed in yeast microsomes under in vitro enzyme assays	Linum usitatissimum

Cloned (Comment)

Organism

two genes LPCAT, LPCAT1 and LPCAT2, containing eight exons and seven introns, respectively, DNA and amino acid seqquence determination and analysis, co-expression of flax genes LPCAT1 and diacylglycerol acyltransferase DGAT1-1 in the yeast quintuple mutant strain H1246 DELTAale1 significantly increasing 18-carbon polyunsaturated fatty acids in triacylglycerol with a concomitant decrease of 18-carbon polyunsaturated fatty acids in phospholipid. The specific activity of flax LPCAT1-catalyzed forward reaction is about 1000 times higher than that of flax DGAT1-catalyzed reaction when the expressed recombinant enzymes are assessed in yeast microsomes under in vitro enzyme assays

Linum usitatissimum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Linum usitatissimum	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine	Linum usitatissimum	phosphatidylcholine is the major site for polyunsaturated fatty acid synthesis	CoA + 1,2-diacyl-sn-glycero-3-phosphocholine	-	r

Organism

Organism	UniProt	Comment	Textmining
Linum usitatissimum	-	isozymes LPCAT1 and LPCAT2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine	phosphatidylcholine is the major site for polyunsaturated fatty acid synthesis	Linum usitatissimum	CoA + 1,2-diacyl-sn-glycero-3-phosphocholine	-	r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine	the reverse reaction of enzyme LPCAT uses phosphatidylcholine as substrate and forms acyl-CoA and lysophosphatidylcholine	Linum usitatissimum	CoA + 1,2-diacyl-sn-glycero-3-phosphocholine	-	r

Synonyms

Synonyms	Comment	Organism
acyl-CoA:lysophosphatidylcholine acyltransferase	-	Linum usitatissimum
LPCAT	-	Linum usitatissimum
LPCAT1	-	Linum usitatissimum
LPCAT2	-	Linum usitatissimum
lysophosphatidylcholine acyltransferase	-	Linum usitatissimum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at, forward and reverse reaction	Linum usitatissimum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at, forward and reverse reaction	Linum usitatissimum

General Information

General Information	Comment	Organism
evolution	the LPCAT genes are highly conserved, suggesting that the duplicated LPCAT genes were derived from a segmental duplication event. The segmental duplication is the result of a recent whole-genome duplication event in flax	Linum usitatissimum
metabolism	the enzyme is involved in the Kennedy pathway for triacylglycerol synthesis, biochemical coupling of isozyme LPCAT1 and the diacylglycerol acyltransferase DGAT1-1-catalyzed reactions, overview. Both enzymes are required for synthesis of triacylgycerol	Linum usitatissimum
additional information	the specific activity of flax LPCAT1-catalyzed forward reaction is about 1000 times higher than that of flax DGAT1-catalyzed reaction when the coexpressed recombinant enzymes are assessed in yeast microsomes under in vitro enzyme assays, overview	Linum usitatissimum
physiological function	phosphatidylcholine is the major site for polyunsaturated fatty acid synthesis. Enzyme lysophosphatidylcholine acyltransferase can transfer polyunsaturated fatty acids on phosphatidylcholine directly into the acyl-CoA pool, making these polyunsaturated fatty acids available for the diacylglycerol acyltransferase (DGAT)-catalyzed reaction for triacylglycerol production, proposed mechanism, overview	Linum usitatissimum