Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.23 extracted from

  • Moessinger, C.; Kuerschner, L.; Spandl, J.; Shevchenko, A.; Thiele, C.
    Human lysophosphatidylcholine acyltransferases 1 and 2 are located in lipid droplets where they catalyze the formation of phosphatidylcholine (2011), J. Biol. Chem., 286, 21330-21339.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in A-431 cell and COS-7 cell Homo sapiens
expression in A-431 cell, COS-7 cell, and Hu-H7 cell Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
lipid droplet in addition to endoplasmic reticulum, both isoforms LCAT1 and LCAT2 localize to lipid droplets, which consist of a core of neutral lipids surrounded by a monolayer of phospholipid, mainly phosphatidylcholine. This dual localization is enabled by the monotopic topology of the enzymes with both termini facing the cytoplasm, irrespective of supplementation with oleic acid. Lipid droplets have the ability to locally synthesize phosphatidylcholine and this activity correlates with the LPCAT1 and -2 expression levels Homo sapiens 5811
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q7L5N7 isoform PCAT2
-
Homo sapiens Q8NF37 isoform PCAT1
-

Source Tissue

Source Tissue Comment Organism Textmining
A-431 cell high expression of both isoforms LCAT1 and LCAT2 Homo sapiens
-
Huh-7 cell low expression of isoform LCAT1 Homo sapiens
-
lung
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
PCAT1
-
 Homo sapiens
PCAT2
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function double knockdown of both isoforms LPCAT1 and LPCAT2 in A-431 cells leads to strong reduction of lipid-droplet-associated enzymic activity Homo sapiens
physiological function double knockdown of both isoforms LPCAT1 and LPCAT2 in A-431 cells or single knockdown of isoform LPCAT1 in Hu-H7 cells leads to strong reduction of lipid-droplet-associated enzymic activity. Reduction of LPCAT1 activity in Hu-H7 cells is associated with a morphological alteration in the lipid droplet pool, the mean lipid droplet size increases, although the amount of stored triacylglycerols remains constant Homo sapiens