Literature summary for 2.3.1.23 extracted from

Jain, S.; Zhang, X.; Khandelwal, P.J.; Saunders, A.J.; Cummings, B.S.; Oelkers, P.
Characterization of human lysophospholipid acyltransferase 3 (2009), J. Lipid Res., 50, 1563-1570.

Search for more literature for 2.3.1.23

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Sf9 insect cell	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0021	-	stearoyl-CoA	37°C, cooperativity: Hill number 3.7	Homo sapiens
0.0051	-	stearoyl-CoA	28°C, cooperativity: Hill number 2.7	Homo sapiens
0.0122	-	oleoyl-CoA	37°C, cooperativity: Hill number 1.6	Homo sapiens
0.0131	-	lysophosphatidylcholine	cooperativity: Hill number 1.7	Homo sapiens
0.0465	-	oleoyl-CoA	28°C, cooperativity: Hill number 2.0	Homo sapiens
0.0558	-	Arachidonoyl-CoA	28°C, cooperativity: Hill number 2.0	Homo sapiens
0.214	-	alpha-linolenoyl-CoA	28°C, no cooperativity: Hill number 1.0	Homo sapiens
0.232	-	stearoyl-CoA	37°C, cooperativity: Hill number 1.4	Homo sapiens
0.245	-	Arachidonoyl-CoA	37°C, cooperativity: Hill number 2.0	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	x * 56000, predicted	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q6P1A2	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293 cell	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.006	-	substrate: stearoyl-CoA, 37°C, cooperativity: Hill number 3.7	Homo sapiens
0.05	-	substrate: stearoyl-CoA, 28°C, cooperativity: Hill number 2.7	Homo sapiens
0.082	-	substrate: arachidonoyl-CoA, 37°C, cooperativity: Hill number 2.0	Homo sapiens
0.087	-	substrate: oleoyl-CoA, 37°C, cooperativity: Hill number 1.6	Homo sapiens
0.268	-	substrate: alpha-linolenoyl-CoA, 28°C, no cooperativity: Hill number 1.0	Homo sapiens
0.323	-	substrate: stearoyl-CoA, 37°C, cooperativity: Hill number 1.4	Homo sapiens
0.486	-	substrate: lysophosphatidylcholine, cooperativity: Hill number 1.7	Homo sapiens
0.529	-	substrate: arachidonoyl-CoA, 28°C, cooperativity: Hill number 2.0	Homo sapiens
0.759	-	substrate: oleoyl-CoA, 28°C, cooperativity: Hill number 2.0	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-palmitoyl-2-lysophosphatidylcholine + alpha-linolenoyl-CoA	-	Homo sapiens	1-palmitoyl-2-alpha-linolenoylphosphatidylcholine + CoA	-	?
1-palmitoyl-2-lysophosphatidylcholine + arachidonoyl-CoA	-	Homo sapiens	1-palmitoyl-2-arachidonoylphosphatidylcholine + CoA	-	?
1-palmitoyl-2-lysophosphatidylcholine + oleoyl-CoA	-	Homo sapiens	1-palmitoyl-2-oleoylphosphatidylcholine + CoA	-	?
1-palmitoyl-2-lysophosphatidylcholine + stearoyl-CoA	-	Homo sapiens	1-palmitoyl-2-stearoylphosphatidylcholine + CoA	-	?
additional information	reduced activity with lysophosphatidylethanolamine and lysophosphatidylserine	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 56000, predicted	Homo sapiens

Synonyms

Synonyms	Comment	Organism
LPCAT3	-	Homo sapiens
lysophosphatidylcholine acyltransferase 3	-	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.005	-	Arachidonoyl-CoA	37°C, cooperativity: Hill number 2.0	Homo sapiens
0.02	-	alpha-linolenoyl-CoA	28°C, no cooperativity: Hill number 1.0	Homo sapiens
0.02	-	stearoyl-CoA	37°C, cooperativity: Hill number 1.4	Homo sapiens
0.04	-	stearoyl-CoA	37°C, cooperativity: Hill number 3.7	Homo sapiens
0.12	-	oleoyl-CoA	37°C, cooperativity: Hill number 1.6	Homo sapiens
0.16	-	Arachidonoyl-CoA	28°C, cooperativity: Hill number 2.0	Homo sapiens
0.16	-	stearoyl-CoA	28°C, cooperativity: Hill number 2.7	Homo sapiens
0.27	-	oleoyl-CoA	28°C, cooperativity: Hill number 2.0	Homo sapiens

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Release 2023.1 (January 2023)