Cloned (Comment) | Organism |
---|---|
LPCAT1, DNA and amino acid sequence determination and analysis | Rattus norvegicus |
LPCAT1, DNA and amino acid sequence determination and analysis, quantitative expression analysis, expression of FLAG-tagged enzyme in CHO-K1 cells, subcellular distribution pattern analysis shows enzyme expression mainly in the endoplasmic reticulum-Golgi, but also in mitochondria, and lysosome of the transfected cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
palmitoyl-CoA | inhibition at higher concentrations of palmitoyl-CoA, possibly due to a detergent effect | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Mus musculus | |
0.0023 | - |
1-palmitoyl-lysophosphatidylcholine | pH 7.4, 30°C, recombinant enzyme | Mus musculus | |
0.003 | - |
palmitoyl-CoA | pH 7.4, 30°C, recombinant enzyme | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme contains 3 transmembrane domains | Mus musculus | 16020 | - |
membrane | the enzyme contains 3 transmembrane domains | Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+ is not required | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, recombinant enzyme, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | the enzyme synthesizes phosphatidylcholine in pulmonary surfactant and plays a pivotal role in respiratory physiology and in membrane biogenesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q3TFD2 | - |
- |
Rattus norvegicus | Q1HAQ0 | Sprague-Dawley rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
alveolar type II cell | - |
Mus musculus | - |
alveolar type II cell | - |
Rattus norvegicus | - |
brain | - |
Mus musculus | - |
epididymis | - |
Mus musculus | - |
lung | - |
Rattus norvegicus | - |
lung | LPCAT1 is predominantly expressed in the lung, in particular in alveolar type II cells, but occurs also in other tissues | Mus musculus | - |
additional information | LPCAT1 shows a wide tissue distribution, overview | Mus musculus | - |
ovary | - |
Mus musculus | - |
placenta | - |
Mus musculus | - |
spleen | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme synthesizes phosphatidylcholine in pulmonary surfactant and plays a pivotal role in respiratory physiology and in membrane biogenesis | Mus musculus | ? | - |
? | |
additional information | LPCAT1 shows a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl- or 1-palmitoyl-LPC as acyl donors and acceptors, respectively | Mus musculus | ? | - |
? | |
palmitoyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine | i.e. 1-palmitoyl-lysophosphatidylcholine | Mus musculus | CoA + dipalmitoylphosphatidylcholine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant enzyme, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
LPCAT1 | - |
Mus musculus |
LPCAT1 | - |
Rattus norvegicus |
lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1 | - |
Mus musculus |
lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 10 | recombinant enzyme | Mus musculus |