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Literature summary for 2.3.1.225 extracted from
- DHHC protein S-acyltransferases use similar ping-pong kinetic mechanisms but display different acyl-CoA specificities (2012), J. Biol. Chem., 287, 7236-7245.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of FLAG- and His6-tagged DHHC2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system | Homo sapiens |
| expression of FLAG- and His6-tagged wild-type and C157S mutant DHHC3 in Spodopterafrugiperda Sf9 cells using the baculovirus transfection system | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C157S | site-directed mutagenesis, inactive mutant | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | acyl-CoAs of 14 carbons and longer inhibit palmitoyl-CoA labeling of both substrate and enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
| additional information | - |
additional information | Michaelis-Menten kinetics | Mus musculus | |
| 0.00084 | - |
[N-myristoylated Gly-Cys-Gly tripeptide]-L-cysteine | pH 7.4, 25°C, recombinant DHHC3 | Homo sapiens |  |
| 0.0013 | - |
[N-myristoylated Gly-Cys-Gly tripeptide]-L-cysteine | pH 7.4, 25°C, recombinant DHHC3 | Mus musculus | |
| 0.0052 | - |
palmitoyl-CoA | pH 7.4, 25°C, recombinant DHHC3 | Homo sapiens | |
| 0.0059 | - |
palmitoyl-CoA | pH 7.4, 25°C, recombinant DHHC3 | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + [protein]-L-cysteine | Homo sapiens | - |
[protein]-S-palmitoyl-L-cysteine + CoA | - |
r | |
| palmitoyl-CoA + [protein]-L-cysteine | Mus musculus | - |
[protein]-S-palmitoyl-L-cysteine + CoA | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | Q8R173 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant FLAG- and His6-tagged DHHC2 from Sf9 cells by nickel and FLAG affinity chromatography to near homogeneity | Homo sapiens |
| recombinant FLAG- and His6-tagged wild-type and C157S mutant DHHC3 from Sf9 cells by nickel and FLAG affinity chromatography to near homogeneity | Mus musculus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA | two-step transfer mechanism, ping-pong kinetic mechanism | Homo sapiens | |
| palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA | two-step transfer mechanism, ping-pong kinetic mechanism | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | DHHC2 has a broad protein substrate specificity. DHHC2 transfers fatty acids from all acyl-CoA chain lengths tested, consistent with it having a broad specificity for long chain acyl-CoAs, and acyl-CoAs of 14 carbons and longer inhibit palmitoyl-CoA labeling of both substrate and enzyme. The acyl-CoA chain length specificity of DHHC enzyme autoacylation parallels substrate specificity, overview | Homo sapiens | ? | - |
? | |
| additional information | DHHC3 has a broad protein substrate specificity, but only myristoyl-, palmitoyl-, and palmioleoyl-CoA are effective, and longer acyl-CoAs compete less well. The acyl-CoA chain length specificity of DHHC enzyme autoacylation parallels substrate specificity, overview | Mus musculus | ? | - |
? | |
| palmitoyl-CoA + [N-myristoylated G-protein alphai1]-L-cysteine | - |
Homo sapiens | [N-myristoylated G-protein alphai1]-S-palmitoyl-L-cysteine + CoA | - |
r | |
| palmitoyl-CoA + [N-myristoylated G-protein alphai1]-L-cysteine | - |
Mus musculus | [N-myristoylated G-protein alphai1]-S-palmitoyl-L-cysteine + CoA | - |
r | |
| palmitoyl-CoA + [N-myristoylated Gly-Cys-Gly tripeptide]-L-cysteine | peptide substrate si tagged via ethylenediamine with fluorescent NBD | Homo sapiens | [N-myristoylated Gly-Cys-Gly tripeptide]-S-palmitoyl-L-cysteine + CoA | - |
r | |
| palmitoyl-CoA + [N-myristoylated Gly-Cys-Gly tripeptide]-L-cysteine | peptide substrate si tagged via ethylenediamine with fluorescent NBD | Mus musculus | [N-myristoylated Gly-Cys-Gly tripeptide]-S-palmitoyl-L-cysteine + CoA | - |
r | |
| palmitoyl-CoA + [protein]-L-cysteine | - |
Homo sapiens | [protein]-S-palmitoyl-L-cysteine + CoA | - |
r | |
| palmitoyl-CoA + [protein]-L-cysteine | - |
Mus musculus | [protein]-S-palmitoyl-L-cysteine + CoA | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHHC protein | - |
Homo sapiens |
| DHHC protein | - |
Mus musculus |
| DHHC protein acyltransferase | - |
Homo sapiens |
| DHHC protein acyltransferase | - |
Mus musculus |
| DHHC2 | - |
Homo sapiens |
| DHHC3 | - |
Mus musculus |
| Pat | - |
Homo sapiens |
| Pat | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
| 25 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
| 7.4 | - |
assay at | Mus musculus |
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Release 2023.1 (January 2023)
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