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Literature summary for 2.3.1.222 extracted from
- PduL is an evolutionarily distinct phosphotransacylase involved in B12-dependent 1,2-propanediol degradation by Salmonella enterica serovar typhimurium LT2 (2007), J. Bacteriol., 189, 1589-1596.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pduL, DNA and amino acid sequence determination and analysis, sequence comparison, expression as His8-tagged protein in Escherichia coli strain BE554 mostly in inclusion bodies | Salmonella enterica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.61 | - |
propanoyl phosphate | reverse reaction, pH and temperature not specified in the publication | Salmonella enterica |  |
| 0.97 | - |
acetyl phosphate | reverse reaction, pH and temperature not specified in the publication | Salmonella enterica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, recombinant His8-tagged PduL, SDS-PAGE | Salmonella enterica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| propanoyl-CoA + phosphate | Salmonella enterica | - |
CoA + propanoyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica | - |
serovar typhimurium LT2, diverse strains, overview, gene pduL | - |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His8-tagged PduL from Escherichia coli strain BE554 soluble fraction by nickel affinity chromatographhy | Salmonella enterica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
wild-type strain, pH and temperature not specified in the publication | Salmonella enterica |
| 50 | - |
recombinant His8-tagged PduL in soluble fraction of the expressing Escherichia coli strain BE554, pH and temperature not specified in the publication | Salmonella enterica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | - |
Salmonella enterica | CoA + acetyl phosphate | - |
r | |
| CoA + acetyl phosphate | PduL-His8 is 3.8fold more active with propionyl phosphate than with acetyl phosphate | Salmonella enterica | acetyl-CoA + phosphate | - |
r | |
| CoA + propanoyl phosphate | PduL-His8 is 3.8fold more active with propionyl phosphate than with acetyl phosphate | Salmonella enterica | propanoyl-CoA + phosphate | - |
r | |
| propanoyl-CoA + phosphate | - |
Salmonella enterica | CoA + propanoyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, recombinant His8-tagged PduL, SDS-PAGE | Salmonella enterica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PduL | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| PduL | - |
Salmonella enterica |
| phosphotransacylase | - |
Salmonella enterica |
| PTAC | - |
Salmonella enterica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.4 | - |
acetyl phosphate | reverse reaction, pH and temperature not specified in the publication | Salmonella enterica | |
| 20.7 | - |
propanoyl phosphate | reverse reaction, pH and temperature not specified in the publication | Salmonella enterica | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | PduL homologues are distributed among at least 49 bacterial species but are absent from the Archaea and Eukarya. Sequence analyses show that PduL is unrelated in amino acid sequence to known phosphotransacylase, PTAC, enzymes | Salmonella enterica |
| malfunction | pduL mutants are unable to ferment 1,2-propanediol and are also impaired for aerobic growth on this compound, overview. Ectopic expression of pduL corrects the growth defects of a pta mutant, the pta gene encodes another PTAC enzyme | Salmonella enterica |
| metabolism | PduL is required in the 1,2-propandiol degradation pathway, overview | Salmonella enterica |
| physiological function | PduL is a phosphotransacylase, PTAC, required for 1,2-propanediol degradation | Salmonella enterica |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.6 | - |
acetyl phosphate | reverse reaction, pH and temperature not specified in the publication | Salmonella enterica | |
| 33.9 | - |
propanoyl phosphate | reverse reaction, pH and temperature not specified in the publication | Salmonella enterica | |
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