Literature summary for 2.3.1.220 extracted from

Morita, H.; Takahashi, Y.; Noguchi, H.; Abe, I.
Enzymatic Formation of Unnatural Aromatic Polyketides by Chalcone Synthase (2000), Biochem. Biophys. Res. Commun., 279, 190-195.

Search for more literature for 2.3.1.220

Organism

Organism	UniProt	Comment	Textmining
Scutellaria baicalensis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3 malonyl-CoA + benzoyl-CoA	-	Scutellaria baicalensis	4 CoA + 2,4,6-trihydroxybenzophenone + 3 CO2	-	?
3 malonyl-CoA + hexanoyl-CoA	-	Scutellaria baicalensis	4 CoA + 4-hydroxy-6-pentyl-2H-pyran-2-one + 4-hydroxy-6-(2-oxoheptyl)-2H-pyran-2-one + 3 CO2	-	?
3 malonyl-CoA + isobutyryl-CoA	-	Scutellaria baicalensis	4 CoA + phloroisobutyrophenone + 3 CO2	-	?
3 malonyl-CoA + isovaleryl-CoA	-	Scutellaria baicalensis	4 CoA + phloroisovalerophenone + 3 CO2	-	?
3 malonyl-CoA + phenylacetyl-CoA	-	Scutellaria baicalensis	4 CoA + 2,4,6-trihydroxyphenylbenzylketone + 3 CO2	-	?
additional information	chalcone synthase, CHS EC 2.3.1.74, shows broad substrate specificity, overview. Both aromatic and aliphatic CoA esters are accepted in the active site of the enzyme as a starter substrate for the complex condensation reaction. CHS converts benzoyl-CoA to phlorobenzophenone, i.e. 2,4,6-trihydroxybenzophenone, along with pyrone by-products, and it converts phenylacetyl-CoA to an unnatural aromatic polyketide, phlorobenzylketone, i.e. 2,4,6-trihydroxyphenylbenzylketone. Scutellaria baicalensis CHS also accepts aliphatic CoA esters, isovaleryl-CoA and isobutyryl-CoA, to produce phloroacylphenones. Hexanoyl-CoA only affords pyrone derivatives without formation of a new aromatic ring	Scutellaria baicalensis	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Scutellaria baicalensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Scutellaria baicalensis

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Release 2023.1 (January 2023)