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Literature summary for 2.3.1.219 extracted from

  • Katsuyama, Y.; Kita, T.; Funa, N.; Horinouchi, S.
    Curcuminoid biosynthesis by two type III polyketide synthases in the herb Curcuma longa (2009), J. Biol. Chem., 284, 11160-11170.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Curcuma longa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic analysis of CURS, overview Curcuma longa
0.085
-
4-coumaroyl-CoA pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA Curcuma longa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O Curcuma longa
-
bisdemethoxycurcumin + CO2 + CoA
-
?
(4-coumaroyl)acetyl-CoA + feruloyl-CoA + H2O Curcuma longa
-
demethoxycurcumin + CO2 + CoA
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA Curcuma longa
-
demethoxycurcumin + CO2 + CoA
-
?

Organism

Organism UniProt Comment Textmining
Curcuma longa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Curcuma longa
-
rhizome
-
Curcuma longa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O
-
Curcuma longa bisdemethoxycurcumin + CO2 + CoA
-
?
(4-coumaroyl)acetyl-CoA + feruloyl-CoA + H2O
-
Curcuma longa demethoxycurcumin + CO2 + CoA
-
?
4-coumaroyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O
-
Curcuma longa CoA + cinnamoyl-4-coumaroylmethane + N-acetylcysteamine
-
?
4-coumaroyl-CoA + feruloylacetyl-CoA
-
Curcuma longa demethoxycurcumin + CO2 + CoA
-
?
cinnamoyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O low activity Curcuma longa CoA + dicinnamoylmethane + N-acetylcysteamine
-
?

Synonyms

Synonyms Comment Organism
curcumin synthase
-
Curcuma longa
CURS
-
Curcuma longa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Curcuma longa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.15
-
4-coumaroyl-CoA pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA Curcuma longa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Curcuma longa

General Information

General Information Comment Organism
evolution the enzyme belongs to the Type III polyketide synthase family Curcuma longa
metabolism the enzyme catalyzes a step in the curcuminoid biosynthesis, pathway overview Curcuma longa
additional information CURS can collaborate with curcumin synthase, DCS, EC 2.3.1.218, which provides the substrate feruloylacetyl-CoA from condensation of feruloyl-CoA and malonyl-CoA, overview Curcuma longa