Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Curcuma longa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of CURS, overview | Curcuma longa | |
0.085 | - |
4-coumaroyl-CoA | pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA | Curcuma longa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O | Curcuma longa | - |
bisdemethoxycurcumin + CO2 + CoA | - |
? | |
(4-coumaroyl)acetyl-CoA + feruloyl-CoA + H2O | Curcuma longa | - |
demethoxycurcumin + CO2 + CoA | - |
? | |
4-coumaroyl-CoA + feruloylacetyl-CoA | Curcuma longa | - |
demethoxycurcumin + CO2 + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curcuma longa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Curcuma longa | - |
rhizome | - |
Curcuma longa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O | - |
Curcuma longa | bisdemethoxycurcumin + CO2 + CoA | - |
? | |
(4-coumaroyl)acetyl-CoA + feruloyl-CoA + H2O | - |
Curcuma longa | demethoxycurcumin + CO2 + CoA | - |
? | |
4-coumaroyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O | - |
Curcuma longa | CoA + cinnamoyl-4-coumaroylmethane + N-acetylcysteamine | - |
? | |
4-coumaroyl-CoA + feruloylacetyl-CoA | - |
Curcuma longa | demethoxycurcumin + CO2 + CoA | - |
? | |
cinnamoyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O | low activity | Curcuma longa | CoA + dicinnamoylmethane + N-acetylcysteamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
curcumin synthase | - |
Curcuma longa |
CURS | - |
Curcuma longa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Curcuma longa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.15 | - |
4-coumaroyl-CoA | pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA | Curcuma longa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Curcuma longa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the Type III polyketide synthase family | Curcuma longa |
metabolism | the enzyme catalyzes a step in the curcuminoid biosynthesis, pathway overview | Curcuma longa |
additional information | CURS can collaborate with curcumin synthase, DCS, EC 2.3.1.218, which provides the substrate feruloylacetyl-CoA from condensation of feruloyl-CoA and malonyl-CoA, overview | Curcuma longa |