Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Curcuma longa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of CURS, overview | Curcuma longa | |
0.018 | - |
feruloyl-CoA | pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA | Curcuma longa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl-CoA + feruloylacetyl-CoA + H2O | Curcuma longa | - |
2 CoA + curcumin + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curcuma longa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Curcuma longa | - |
rhizome | - |
Curcuma longa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamoyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O | low activity | Curcuma longa | CoA + dicinnamoylmethane + N-acetylcysteamine | - |
? | |
feruloyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O | - |
Curcuma longa | CoA + cinnamoylferuloylmethane + N-acetylcysteamine + CO2 | - |
? | |
feruloyl-CoA + feruloylacetyl-CoA + H2O | - |
Curcuma longa | 2 CoA + curcumin + CO2 | - |
? | |
feruloyl-CoA + feruloylacetyl-CoA + H2O | feruloyl-CoA is the preferred starter substrate | Curcuma longa | 2 CoA + curcumin + CO2 | product identification by LC-ESI MS/MS | ? | |
additional information | the enzyme can also convert diketide-CoA esters into 2-oxoacids and CoA, which are then converted into curcuminoids. For activity assays, cinnamoyl-CoA, 4-coumaroyl-CoA, or feruloyl-CoA are used as starter substrates and malonyl-CoA or cinnamoyl-diketide-N-acetylcysteamine as extender substrates, cf. EC 2.3.1.218 and EC 2.31.219 | Curcuma longa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
curcumin synthase | - |
Curcuma longa |
CURS | - |
Curcuma longa |
CURS1 | - |
Curcuma longa |
CURS2 | - |
Curcuma longa |
CURS3 | - |
Curcuma longa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Curcuma longa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0183 | - |
feruloyl-CoA | pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA | Curcuma longa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Curcuma longa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the Type III polyketide synthase family | Curcuma longa |
metabolism | the enzyme catalyzes a step in the curcuminoid biosynthesis, pathway overview | Curcuma longa |
additional information | CURS can collaborate with curcumin synthase, DCS, EC 2.3.1.218, which provides the substrate feruloylacetyl-CoA from condensation of feruloyl-CoA and malonyl-CoA, overview | Curcuma longa |