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Literature summary for 2.3.1.216 extracted from

  • Abe, I.
    Engineering of plant polyketide biosynthesis (2008), Chem. Pharm. Bull., 56, 1505-1514.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis Aloe arborescens

Protein Variants

Protein Variants Comment Organism
F80A/Y82A/M207G site-directed mutagenesis, the total cavity volume of the F80A/Y82A/M207G triple mutant is 4fold larger than that of the wild-type pentaketide chromone synthase. The mutant not only catalyzes the iterative condensation of nine molecules of malonyl-CoA, to produce anovel nonaketide naphthopyrone, but also alters the mechanism of the cyclization to produce the angular naphthopyrone with a fused tricyclic ring system Aloe arborescens
M207G site-directed mutagenesis, the pentaketide chromone synthase M207G mutant no longer produces the pentaketide chromone but instead efficiently catalyzes sequential condensations of eight molecules of malonyl-CoA to produce a 1:4 mixture of the octaketides SEK4/SEK4b. The pentaketide-producing pentaketide chromone synthaseis thus transformed into an octaketide synthase by the single-amino acid replacement Aloe arborescens
additional information oktaketide synthase, EC 2.3.1.-, and pentaketide chromone synthase, EC 2.3.1.216, are not functionally interconvertible by the single amino acid switch at residue 207 Aloe arborescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, about, sequence calculation Aloe arborescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5 malonyl-CoA Aloe arborescens
-
5 CoA + 5,7-dihydroxy-2-methyl-4H-chromen-4-one + 5 CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Aloe arborescens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5 malonyl-CoA
-
Aloe arborescens 5 CoA + 5,7-dihydroxy-2-methyl-4H-chromen-4-one + 5 CO2 + H2O
-
?
additional information recombinant pentaketide chromone synthasedoes not produce chalcone from 4-coumaroyl-CoA, but instead efficiently accepts malonyl-CoA as a sole substrate to carry out iterative condensations of five molecules of malonyl-CoA, producing a pentaketide, 5,7-dihydroxy-2-methylchromone. Recombinant pentaketide chromone synthaseaccepts acetyl-CoA, resulting from decarboxylation of malonyl-CoA, as a starter substrate Aloe arborescens ?
-
?

Subunits

Subunits Comment Organism
? x * 44000, about, sequence calculation Aloe arborescens

Synonyms

Synonyms Comment Organism
PCS
-
Aloe arborescens
pentaketide chromone synthase
-
Aloe arborescens

General Information

General Information Comment Organism
evolution pentaketide chromone synthase is a plant-specific type III polyketide synthase that belongs to the chalcone synthase superfamily of type III polyketide synthases and grouped with other non-chalcone forming enzymes Aloe arborescens
additional information the critical active-site residue 197, and the catalytic triad, Cys164, His303, and Asn336, are conserved in type III polyketide synthases, while the residues lining the active-site are exchanged to M197, L256, V338 (numbering in Madia sativa CHS). Active-site architecture of pentaketide chromone synthase compared to other type III polyketide synthases, crystal structure and hmology modeling, overview Aloe arborescens