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Literature summary for 2.3.1.215 extracted from

  • Rinaldo, A.R.; Cavallini, E.; Jia, Y.; Moss, S.M.; McDavid, D.A.; Hooper, L.C.; Robinson, S.P.; Tornielli, G.B.; Zenoni, S.; Ford, C.M.; Boss, P.K.; Walker, A.R.
    A grapevine anthocyanin acyltransferase, transcriptionally regulated by VvMYBA, can produce most acylated anthocyanins present in grape skins (2015), Plant Physiol., 169, 1897-1916.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.215

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vitis vinifera

Protein Variants

Protein Variants Comment Organism
additional information protein sequence changes and gene expression patterns of 3AT correlate with the absence of acylated anthocyanins in cv Pinot Noir and pale-fruited cv Cabernet Sauvignon mutants Vitis vinifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 coumaroyl-CoA pH 6.5, 30°C Vitis vinifera
0.0041
-
 caffeoyl-CoA pH 6.5, 30°C Vitis vinifera
0.0169
-
 acetyl-CoA pH 6.5, 30°C Vitis vinifera
0.064
-
 cyanidin 3-O,5-O-beta-D-diglucoside pH 6.5, 30°C Vitis vinifera
0.079
-
 peonidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
0.098
-
 malvidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
0.506
-
 cyanidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
0.588
-
 malonyl-CoA pH 6.5, 30°C Vitis vinifera
0.765
-
 delphinidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera

Organism

Organism UniProt Comment Textmining
Vitis vinifera D7TU67
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanidin 3-O,5-O-beta-D-diglucoside + acetyl-CoA
-
 Vitis vinifera CoA + cyanidin-3-O-(6-O-acetyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
 ?
cyanidin 3-O-beta-D-glucoside + acetyl-CoA
-
 Vitis vinifera CoA + cyanidin 3-O-(6-O-acetyl)-beta-D-glucoside
-
 ?
delphinidin 3-O-beta-D-glucoside + acetyl-CoA
-
 Vitis vinifera CoA + delphinidin 3-O-(6-O-acetyl)-beta-D-glucoside
-
 ?
malvidin 3-O-beta-D-glucoside + acetyl-CoA
-
 Vitis vinifera CoA + malvidin 3-O-(6-O-acetyl)-beta-D-glucoside
-
 ?
malvidin 3-O-beta-D-glucoside + caffeoyl-CoA
-
 Vitis vinifera CoA + malvidin 3-O-(6-O-caffeoyl)-beta-D-glucoside
-
 ?
malvidin 3-O-beta-D-glucoside + coumaroyl-CoA
-
 Vitis vinifera CoA + malvidin 3-O-(6-O-coumaroyl)-beta-D-glucoside
-
 ?
malvidin 3-O-beta-D-glucoside + malonyl-CoA
-
 Vitis vinifera CoA + malvidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
additional information no substrate: cyanidin 3-rutinoside Vitis vinifera ?
-
 ?
peonidin 3-O-beta-D-glucoside + acetyl-CoA
-
 Vitis vinifera CoA + peonidin 3-O-(6-O-acetyl)-beta-D-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
3AT
-
 Vitis vinifera
anthocyanidin 3-O-glucoside 6''-O-acyltransferase
-
 Vitis vinifera
VIT_03s0017g00870
-
 Vitis vinifera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 coumaroyl-CoA pH 6.5, 30°C Vitis vinifera
0.18
-
 cyanidin 3-O,5-O-beta-D-diglucoside pH 6.5, 30°C Vitis vinifera
0.29
-
 caffeoyl-CoA pH 6.5, 30°C Vitis vinifera
0.92
-
 malonyl-CoA pH 6.5, 30°C Vitis vinifera
1.11
-
 acetyl-CoA pH 6.5, 30°C Vitis vinifera
13.92
-
 malvidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
14.2
-
 peonidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
67.89
-
 delphinidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
77
-
 cyanidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera

Expression

Organism Comment Expression
Vitis vinifera transcription factor MYBA is a positive regulator of the later stages of anthocyanin synthesis, modification, and transport in cv Shiraz. Anthocyanidin 3-O-glucoside 6''-O-acyltransferase is upregulated by MYBA additional information

General Information

General Information Comment Organism
physiological function constitutive expression of 3AT in tobacco results in the production of acylated anthocyanins Vitis vinifera

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6
-
 malonyl-CoA pH 6.5, 30°C Vitis vinifera
3
-
 cyanidin 3-O,5-O-beta-D-diglucoside pH 6.5, 30°C Vitis vinifera
70
-
 caffeoyl-CoA pH 6.5, 30°C Vitis vinifera
80
-
 acetyl-CoA pH 6.5, 30°C Vitis vinifera
90
-
 delphinidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
100
-
 coumaroyl-CoA pH 6.5, 30°C Vitis vinifera
150
-
 cyanidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
150
-
 malvidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
180
-
 peonidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera