Cloned (Comment) | Organism |
---|---|
RiPKS4, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)pLYsS | Rubus idaeus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA + H2O | Rubus idaeus | - |
2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rubus idaeus | B0LDU5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant RiPKS4 from Escherichia coli strain BL21(DE3)pLYsS | Rubus idaeus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | transcript of RiPKS4 accumulates at a lower level during fruit development in comparison to RiPKS1, the gene transcript is not detected during the early stage 1 for RiPKS4 of fruit development | Rubus idaeus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA + H2O | - |
Rubus idaeus | 2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? | |
5-hydroxyferuloyl-CoA + malonyl-CoA + H2O | - |
Rubus idaeus | ? | - |
? | |
caffeoyl-CoA + malonyl-CoA + H2O | - |
Rubus idaeus | ? | - |
? | |
cinnamoyl-CoA + malonyl-CoA + H2O | - |
Rubus idaeus | ? | - |
? | |
feruloyl-CoA + malonyl-CoA + H2O | - |
Rubus idaeus | 2 CoA + (3E)-4-(4-hydroxy-3-methoxyphenyl)but-3-en-2-one + 2 CO2 | - |
? | |
additional information | RiPKS4 is a bifunctional polyketide synthase producing both 4-hydroxybenzalacetone and naringenin chalcone, the latter by reaction of chalcone synthase, EC 2.3.1.74. The recombinant RiPKS4 protein, like the native protein from raspberry fruits accepts 4-coumaryl-CoA and ferulyl-CoA as starter substrates and catalyzes the formation of both naringenin chalcone, 4-hydroxy-benzalacetone, and 3-methoxy-4-hydroxy-benzalacetone. The activity of RiPKS4 is higher with ferulyl-CoA than with 4-coumaryl-CoA | Rubus idaeus | ? | - |
? | |
sinapoyl-CoA + malonyl-CoA + H2O | - |
Rubus idaeus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RiPKS4 | - |
Rubus idaeus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a plant-specific type III polyketide synthase of the chalcone synthase (CHS) superfamily, amino acids C164, F215, H303 and N336 are conserved | Rubus idaeus |