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Literature summary for 2.3.1.212 extracted from

  • Zheng, D.; Hrazdina, G.
    Molecular and biochemical characterization of benzalacetone synthase and chalcone synthase genes and their proteins from raspberry (Rubus idaeus L.) (2008), Arch. Biochem. Biophys., 470, 139-145.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
RiPKS4, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)pLYsS Rubus idaeus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-coumaroyl-CoA + malonyl-CoA + H2O Rubus idaeus
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2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
?

Organism

Organism UniProt Comment Textmining
Rubus idaeus B0LDU5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant RiPKS4 from Escherichia coli strain BL21(DE3)pLYsS Rubus idaeus

Source Tissue

Source Tissue Comment Organism Textmining
fruit transcript of RiPKS4 accumulates at a lower level during fruit development in comparison to RiPKS1, the gene transcript is not detected during the early stage 1 for RiPKS4 of fruit development Rubus idaeus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + malonyl-CoA + H2O
-
Rubus idaeus 2 CoA + 4-hydroxybenzalacetone + 2 CO2
-
?
5-hydroxyferuloyl-CoA + malonyl-CoA + H2O
-
Rubus idaeus ?
-
?
caffeoyl-CoA + malonyl-CoA + H2O
-
Rubus idaeus ?
-
?
cinnamoyl-CoA + malonyl-CoA + H2O
-
Rubus idaeus ?
-
?
feruloyl-CoA + malonyl-CoA + H2O
-
Rubus idaeus 2 CoA + (3E)-4-(4-hydroxy-3-methoxyphenyl)but-3-en-2-one + 2 CO2
-
?
additional information RiPKS4 is a bifunctional polyketide synthase producing both 4-hydroxybenzalacetone and naringenin chalcone, the latter by reaction of chalcone synthase, EC 2.3.1.74. The recombinant RiPKS4 protein, like the native protein from raspberry fruits accepts 4-coumaryl-CoA and ferulyl-CoA as starter substrates and catalyzes the formation of both naringenin chalcone, 4-hydroxy-benzalacetone, and 3-methoxy-4-hydroxy-benzalacetone. The activity of RiPKS4 is higher with ferulyl-CoA than with 4-coumaryl-CoA Rubus idaeus ?
-
?
sinapoyl-CoA + malonyl-CoA + H2O
-
Rubus idaeus ?
-
?

Synonyms

Synonyms Comment Organism
RiPKS4
-
Rubus idaeus

General Information

General Information Comment Organism
evolution the enzyme is a plant-specific type III polyketide synthase of the chalcone synthase (CHS) superfamily, amino acids C164, F215, H303 and N336 are conserved Rubus idaeus