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Literature summary for 2.3.1.21 extracted from

  • Relat, J.; Pujol-Vidal, M.; Haro, D.; Marrero, P.F.
    A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme (2009), FEBS J., 276, 210-218.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Homo sapiens
expressed in Pichia pastoris Sus scrofa

Protein Variants

Protein Variants Comment Organism
D17E pig protein: Glu in this position Homo sapiens
E17D human protein: Asp in this position Sus scrofa
additional information deletion of residues 1-18, and deletion of residues 1-28, human-pig chimeras Sus scrofa
additional information pig-human chimeras Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
malonyl-CoA
-
Homo sapiens
malonyl-CoA
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1976
-
L-carnitine wild-type protein, mitochondrial preparation Sus scrofa
0.6059
-
L-carnitine E17D mutant protein, mitochondrial preparation Sus scrofa
0.683
-
L-carnitine wild-type protein, mitochondrial preparation Homo sapiens
0.7695
-
L-carnitine D17E mutant protein, mitochondrial preparation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00279
-
wild-type protein, mitochondrial preparation Sus scrofa
0.00416
-
chimeric protein: residues 1-128 of human enzyme recombined with pig enzyme, mitochondrial preparation Sus scrofa
0.00443
-
wild-type protein, mitochondrial preparation Homo sapiens
0.00449
-
chimeric protein: residues 1-50 of human enzyme recombined with pig enzyme, mitochondrial preparation Sus scrofa
0.00942
-
protein with deletion of residue 1-28, mitochondrial preparation Sus scrofa
0.00943
-
chimeric protein: residues 1-50 of pig enzyme recombined with human enzyme, mitochondrial preparation Homo sapiens
0.01059
-
chimeric protein: residues 1-128 of pig enzyme recombined with human enzyme, mitochondrial preparation Homo sapiens
0.01528
-
protein with deletion of residue 1-18, mitochondrial preparation Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-carnitine + palmitoyl-CoA
-
Homo sapiens L-palmitoylcarnitine + CoA
-
?
L-carnitine + palmitoyl-CoA
-
Sus scrofa L-palmitoylcarnitine + CoA
-
?

Synonyms

Synonyms Comment Organism
carnitine palmitoyltransferase 1B
-
Homo sapiens
carnitine palmitoyltransferase 1B
-
Sus scrofa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000096
-
wild-type protein, 1 mM carnitine, mitochondrial preparation Homo sapiens malonyl-CoA
0.000117
-
wild-type protein, 0.683 mM carnitine, mitochondrial preparation Homo sapiens malonyl-CoA
0.00019
-
chimeric protein: residues 1-50 of pig enzyme recombined with human enzyme, mitochondrial preparation Homo sapiens malonyl-CoA
0.000246
-
D17E mutant protein, 0.770 mM carnitine, mitochondrial preparation Homo sapiens malonyl-CoA
0.000279
-
D17E mutant protein, 1 mM carnitine, mitochondrial preparation Homo sapiens malonyl-CoA
0.000284
-
E17D mutant protein, 0.606 mM carnitine, mitochondrial preparation Sus scrofa malonyl-CoA
0.000297
-
E17D mutant protein, 1 mM carnitine, mitochondrial preparation Sus scrofa malonyl-CoA
0.000325
-
chimeric protein: residues 1-128 of pig enzyme recombined with human enzyme, mitochondrial preparation Homo sapiens malonyl-CoA
0.000359
-
chimeric protein: residues 1-128 of human enzyme recombined with pig enzyme, mitochondrial preparation Sus scrofa malonyl-CoA
0.000457
-
chimeric protein: residues 1-50 of human enzyme recombined with pig enzyme, mitochondrial preparation Sus scrofa malonyl-CoA
0.00055
-
wild-type protein, 0.198 mM carnitine, mitochondrial preparation Sus scrofa malonyl-CoA
0.000804
-
wild-type protein, 1 mM carnitine, mitochondrial preparation Sus scrofa malonyl-CoA
0.03556
-
protein with deletion of residue 1-18, mitochondrial preparation Sus scrofa malonyl-CoA
0.03919
-
protein with deletion of residue 1-28, mitochondrial preparation Sus scrofa malonyl-CoA