Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant isozymes M-CPT I in Pichia pastoris strain GS115 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A305C | site-directed mutagenesis, the revertant mutant shows about wild-type activity | Homo sapiens |
C305A | site-directed mutagenesis, inactive mutant | Homo sapiens |
C448A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
C504A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
C526A | site-directed mutagenesis, the mutant shows unaltered catalytic activity but increased sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
C548S | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
C562A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
C586A | site-directed mutagenesis, the mutant shows unaltered catalytic activity but reduced sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
C608A | site-directed mutagenesis, the mutant shows unaltered catalytic activity and sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
C659A | site-directed mutagenesis, the mutant shows unaltered catalytic activity but increased sensitivity to malonyl-CoA compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
malonyl-CoA | IC50: 0.0005 mM for the recombinant wild-type enzyme, IC50: 0.00052 mM for the recombinant revertant A305C, IC50 values of recombinant mutant isozymes M-CPT I, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
palmitoyl-CoA | pH 8.0, 37°C, recombinant revertant A305C | Homo sapiens | |
0.073 | - |
palmitoyl-CoA | pH 8.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.824 | - |
L-carnitine | pH 8.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.92 | - |
L-carnitine | pH 8.0, 37°C, recombinant revertant A305C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-carnitine | Homo sapiens | CPT I catalyzes the conversion of long-chain fatty acyl-CoAs to acyl carnitines in the presence of L-carnitine, a rate-limiting step in the transport of long-chain fatty acids from the cytoplasm to the mitochondrial matrix | CoA + L-palmitoylcarnitine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme M-CPT I | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
palmitoyl-CoA + L-carnitine = CoA + L-palmitoylcarnitine | the catalytic triad is composed of Cys305, His473, and Asp454, with Cys305 serving as a probable nucleophile, thus acting as a site for covalent attachment of the acyl molecule and formation of a stable acyl-enzyme intermediate, mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | isozyme M-CPT I | Homo sapiens | - |
muscle | isozyme M-CPT I | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of mutant enzymes | Homo sapiens |
0.0028 | - |
recombinant revertant A305C | Homo sapiens |
0.0029 | - |
recombinant wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-carnitine | - |
Homo sapiens | CoA + L-palmitoylcarnitine | - |
? | |
palmitoyl-CoA + L-carnitine | CPT I catalyzes the conversion of long-chain fatty acyl-CoAs to acyl carnitines in the presence of L-carnitine, a rate-limiting step in the transport of long-chain fatty acids from the cytoplasm to the mitochondrial matrix | Homo sapiens | CoA + L-palmitoylcarnitine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
muscle carnitine palmitoyltransferase I | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0005 | - |
IC50: 0.0005 mM for the recombinant wild-type enzyme | Homo sapiens | malonyl-CoA | |
0.00052 | - |
IC50: 0.00052 mM for the recombinant revertant A305C, IC50 values of recombinant mutant isozymes M-CPT I overview | Homo sapiens | malonyl-CoA |