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Literature summary for 2.3.1.21 extracted from

  • Zhu, H.; Shi, J.; Treber, M.; Dai, J.; Arvidson, D.N.; Woldegiorgis, G.
    Substitution of glutamate-3, valine-19, leucine-23, and serine-24 with alanine in the N-terminal region of human heart muscle carnitine palmitoyltransferase I abolishes malonyl CoA inhibition and binding (2003), Arch. Biochem. Biophys., 413, 67-74.
    View publication on PubMed
Search for more literature for 2.3.1.21

Protein Variants

Protein Variants Comment Organism
DELTA1-18/V19A/L23A/S24A 143fold increase in IC50 for malonyl-CoA, 1.4fold decrease in KM-value for carnitine, 4fold increase in Km-value for palmitoyl-CoA Homo sapiens
E3A 57fold increase in IC50 for malonyl-CoA, 1.5fold decrease in KM-value for carnitine, 1.2fold increase in Km-value for palmitoyl-CoA Homo sapiens
E3A/H5A/V19A/L23A/S24A 97fold increase in IC50 for malonyl-CoA, 1.4fold decrease in KM-value for carnitine, 11fold decrease in Km-value for palmitoyl-CoA Homo sapiens
E3A/V19A/L23A/S24A 143fold increase in IC50 for malonyl-CoA, 1.3fold decrease in KM-value for carnitine, 8.7fold decrease in Km-value for palmitoyl-CoA Homo sapiens
H5A 2.1fold increase in IC50 for malonyl-CoA, 1.1fold increase in KM-value for carnitine, 1.7fold decrease in Km-value for palmitoyl-CoA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
malonyl-CoA Glu3, Leu23, and Ser24 in M-CPTI are important for malonyl-CoA inhibition and binding but not for calalysis. IC50: 0.00007 mM for wild-type enzyme, 0.01 mM for mutant enzyme E3A, 0.00015 for mutant enzyme H5A, 0.01 mM for mutant enzyme E3A/V19A/L23A/S24A, 0.0068 mM for mutant enzyme E3A/H5A/V19A/L23A/S24A Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0062
-
 palmitoyl-CoA mutant enzyme E3A/H5A/V19A/L23A/S24A Homo sapiens
0.0078
-
 palmitoyl-CoA mutant enzyme E3A/V19A/L23A/S24A Homo sapiens
0.0404
-
 palmitoyl-CoA mutant enzyme H5A Homo sapiens
0.0679
-
 palmitoyl-CoA wild-type enzyme Homo sapiens
0.0823
-
 palmitoyl-CoA mutant enzyme E3A Homo sapiens
0.273
-
 palmitoyl-CoA mutant enzyme DELTA1-18/V19A/L23A/S24A Homo sapiens
0.617
-
 L-carnitine mutant enzyme E3A Homo sapiens
0.63
-
 L-carnitine mutant enzyme DELTA1-18/V19A/L23A/S24A Homo sapiens
0.652
-
 L-carnitine mutant enzyme E3A/H5A/V19A/L23A/S24A Homo sapiens
0.706
-
 L-carnitine mutant enzyme E3A/V19A/L23A/S24A Homo sapiens
0.913
-
 L-carnitine wild-type enzyme Homo sapiens
0.968
-
 L-carnitine mutant enzyme H5A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + L-carnitine
-
 Homo sapiens CoA + L-palmitoylcarnitine
-
 ?

Synonyms

Synonyms Comment Organism
carnitine palmitoyltransferase I
-
 Homo sapiens
M-CPTI
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00007
-
 Glu3, Leu23, and Ser24 in M-CPTI are important for malonyl-CoA inhibition and binding but not for calalysis. IC50: 0.00007 mM for wild-type enzyme, 0.01 mM for mutant enzyme E3A, 0.00015 for mutant enzyme H5A, 0.01 mM for mutant enzyme E3A/V19A/L23A/S24A, Homo sapiens malonyl-CoA