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Literature summary for 2.3.1.21 extracted from
- Reconstitution of purified, active and malonyl-CoA-sensitive rat liver carnitine palmitoyltransferase I: relationship between membrane environment and malonyl-CoA sensitivity (2000), Biochem. J., 349, 179-187.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged L-CPT I in Pichia pastoris | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| malonyl-CoA | CPT I; membrane or micelle composition and properties influence the sensitivity of CPT I to inhibition; regulatory role in vivo | Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
palmitoyl-CoA | purified recombinant L-CPT I, reconstituted in liposomes | Rattus norvegicus | |
| 0.039 | - |
L-carnitine | purified recombinant L-CPT I, reconstituted in liposomes | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | 2 isoforms of CPT I, L-CPT I and M-CPT I | Rattus norvegicus | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + L-carnitine | Rattus norvegicus | enzyme has a key function in regulation of fatty acid beta-oxidation | CoA + L-acylcarnitine | - |
? | |
| acyl-CoA + L-carnitine | Rattus norvegicus | CPT I, involved in regulation of fatty acid oxidation | CoA + L-acylcarnitine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
enzyme form CPT I | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged L-CPT I from Pichia pastoris | Rattus norvegicus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution of recombinant L-CPT I from Pichia pastoris in phospholipids after purification with detergent extraction | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | L-CPT I | Rattus norvegicus | - |
| liver | M-CPT I | Rattus norvegicus | - |
| liver | CPT I | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.473 | - |
purified recombinant L-CPT I, reconstituted in liposomes | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + L-carnitine | - |
Rattus norvegicus | CoA + L-acylcarnitine | - |
? | |
| acyl-CoA + L-carnitine | enzyme has a key function in regulation of fatty acid beta-oxidation | Rattus norvegicus | CoA + L-acylcarnitine | - |
? | |
| acyl-CoA + L-carnitine | CPT I, involved in regulation of fatty acid oxidation | Rattus norvegicus | CoA + L-acylcarnitine | - |
? | |
| palmitoyl-CoA + L-carnitine | - |
Rattus norvegicus | CoA + L-palmitoylcarnitine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| 7.2 | - |
assay at | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | 8.8 | L-CPT I | Rattus norvegicus |
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Release 2023.1 (January 2023)
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