Crystallization (Comment) | Organism |
---|---|
apo-crystal structures of the acetyltransferase domain ATD and in complex with AcCoA. The N-terminal section (Asn199-Leu285) comprises a binding pocket for the UDP-4-amino sugar substrate through a beta-alpha-beta-alpha-beta Rossman fold motif. The C-terminus (Pro286-Leu403) is composed of a left-handed beta-helix motif that, in conjunction with an adjacent PglB-ATD protomer in the trimeric state, forms an extended cleft that is utilized for AcCoA binding | Neisseria gonorrhoeae |
compared to isoforms PglD and PglB, WeeI contains an additional loop (Gln174-Pro180) that forms the substrate binding pocket near the pyranose moiety. Residue Gln174 seems to be critical for catalysis. When aligned to the PglD UDP-4-amino structure, this position is analogous to Asn162 that interacts with the carbonyl oxygen of the pyranose C2-acetyl group. In the WeeI apo-structure, Gln174 is within 5 A of the catalytic base His138 and 3.6 A of the AcCoA thioester | Acinetobacter baumannii |
Protein Variants | Comment | Organism |
---|---|---|
D332A | active site mutant, strong loss of activity | Neisseria gonorrhoeae |
F13A | active site mutant, strong loss of activity | Acinetobacter baumannii |
H210F | active site mutant, strong loss of activity | Neisseria gonorrhoeae |
Q174A | active site mutant, strong loss of activity | Acinetobacter baumannii |
Q369A | active site mutant, strong loss of activity | Neisseria gonorrhoeae |
Q370A | active site mutant, 50% loss of activity | Neisseria gonorrhoeae |
T176A | active site mutant, strong loss of activity | Acinetobacter baumannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant Q370A, pH 7.4, 25°C | Neisseria gonorrhoeae | |
0.097 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant Q369A, pH 7.4, 25°C | Neisseria gonorrhoeae | |
0.099 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | wild-type, pH 7.4, 25°C | Neisseria gonorrhoeae | |
0.32 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant H210F, pH 7.4, 25°C | Neisseria gonorrhoeae | |
0.56 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant D332A, pH 7.4, 25°C | Neisseria gonorrhoeae | |
2.42 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant Q174A, pH 7.4, 25°C | Acinetobacter baumannii | |
2.52 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | wild-type, pH 7.4, 25°C | Acinetobacter baumannii | |
4.51 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant F13A, pH 7.4, 25°C | Acinetobacter baumannii | |
4.6 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant T176A, pH 7.4, 25°C | Acinetobacter baumannii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | B0V6J7 | - |
- |
Acinetobacter baumannii AYE | B0V6J7 | - |
- |
Neisseria gonorrhoeae | Q5FAE1 | - |
- |
Neisseria gonorrhoeae FA 1090 | Q5FAE1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | - |
Neisseria gonorrhoeae | CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose | - |
? | |
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | - |
Acinetobacter baumannii | CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose | - |
? | |
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | - |
Acinetobacter baumannii AYE | CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose | - |
? | |
acetyl-CoA + UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | - |
Neisseria gonorrhoeae FA 1090 | CoA + UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ABAYE3807 | - |
Acinetobacter baumannii |
NGO_0085 | - |
Neisseria gonorrhoeae |
WeeI | - |
Acinetobacter baumannii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1200 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant H210F, pH 7.4, 25°C | Neisseria gonorrhoeae | |
1900 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant Q174A, pH 7.4, 25°C | Acinetobacter baumannii | |
3000 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant Q370A, pH 7.4, 25°C | Neisseria gonorrhoeae | |
3600 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant D332A, pH 7.4, 25°C | Neisseria gonorrhoeae | |
5600 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant Q369A, pH 7.4, 25°C | Neisseria gonorrhoeae | |
72000 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | wild-type, pH 7.4, 25°C | Neisseria gonorrhoeae | |
82000 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant F13A, pH 7.4, 25°C | Acinetobacter baumannii | |
330000 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant T176A, pH 7.4, 25°C | Acinetobacter baumannii | |
510000 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | wild-type, pH 7.4, 25°C | Acinetobacter baumannii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
800 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant Q174A, pH 7.4, 25°C | Acinetobacter baumannii | |
3700 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant H210F, pH 7.4, 25°C | Neisseria gonorrhoeae | |
6500 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant D332A, pH 7.4, 25°C | Neisseria gonorrhoeae | |
18000 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant F13A, pH 7.4, 25°C | Acinetobacter baumannii | |
58000 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant Q369A, pH 7.4, 25°C | Neisseria gonorrhoeae | |
71000 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant T176A, pH 7.4, 25°C | Acinetobacter baumannii | |
200000 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | wild-type, pH 7.4, 25°C | Acinetobacter baumannii | |
340000 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | mutant Q370A, pH 7.4, 25°C | Neisseria gonorrhoeae | |
730000 | - |
UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose | wild-type, pH 7.4, 25°C | Neisseria gonorrhoeae |