Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Bordetella petrii |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method. The enzyme complexed with acetyl-CoA and UDP is crystallized at pH 8.0 in the presence of 100 mM HEPPS, 16-20% (w/v) poly(ethylene glycol) 3400 and 210 mM tetramethylammonium chloride. The enzyme bound to CoA and UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate is crystallized using 17% (w/v) poly(ethylene glycol) 3400, 300 mM NaCl, 100 mM HEPPS (pH 8.0), 210 mM tetramethylammonium chloride | Bordetella petrii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella petrii | A9IH93 | - |
- |
Bordetella petrii ATCC BAA-461 | A9IH93 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Bordetella petrii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | - |
Bordetella petrii | CoA + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | - |
? | |
acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate | - |
Bordetella petrii ATCC BAA-461 | CoA + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | x-ray crystallography | Bordetella petrii |
Synonyms | Comment | Organism |
---|---|---|
WlbB | - |
Bordetella petrii |